期刊
MOLECULAR THERAPY-NUCLEIC ACIDS
卷 5, 期 -, 页码 -出版社
CELL PRESS
DOI: 10.1038/mtna.2016.95
关键词
CRISPR; crRNA; Cas9; genome editing; human cells; RGEN
资金
- Science and Technology Development Planing Program of Shandong Province [2014GGH218029]
- National Science Foundation of China [81572559, 31500027, 41106151]
- COMRA program [DY125-15-R-01]
Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clearly detailing a 20-nt targeting sequence inside its directed RNA. We discuss the most recent advancements in the molecular basis of the type II CRISPR/Cas system and encapsulate applications and elements affecting its use in human cells. We also propose possible applications covering its uses ranging from basic science to implementation in the clinic.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据