4.3 Article

Standard Operating Procedures for Biospecimen Collection, Processing, and Storage From the Type 1 Diabetes in Acute Pancreatitis Consortium

期刊

PANCREAS
卷 51, 期 6, 页码 593-597

出版社

LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MPA.0000000000002077

关键词

laboratory protocol; pancreas; immunology; type 1 diabetes; biosamples; biorepository; AP; acute pancreatitis; CPDPC; Chronic Pancreatitis Diabetes Pancreas Cancer Consortium; DCC; data coordinating center; DKCR; NIDDK Central Repository; DREAM; Diabetes RElated to Acute pancreatitis and its Mechanisms; LN; liquid nitrogen; NIDDK; National Institute of Diabetes and Digestive and Kidney Diseases; PBMC; peripheral blood mononuclear cells; SOP; standard operating procedures; ST; sample tracking module; T1DAPC; Type 1 Diabetes in Acute Pancreatitis Consortium

资金

  1. National Institute ofDiabetes and Digestive and Kidney Diseases (NIDDK) [U01 DK127367, U01 DK127377, U01 DK127384, U01 DK127388, U01 DK127392, U01 DK127395, U01 DK127400, U01 DK127403, U01 DK 127404, U01 DK127382, U01 DK127378]

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Differences in methods for biospecimen collection, processing, and storage can result in variability and error. Therefore, establishing best practices for standard operating procedures is crucial for successful discovery, development, and validation of disease biomarkers. This article describes the standard operating procedures implemented for biospecimen collection in the DREAM Study.
Differences in methods for biospecimen collection, processing, and storage can yield considerable variability and error. Therefore, best practices for standard operating procedures are critical for successful discovery, development, and validation of disease biomarkers. Here, we describe standard operating procedures developed for biospecimen collection during the DREAM (Diabetes RElated to Acute pancreatitis and its Mechanisms) Study within the Type 1 Diabetes in Acute Pancreatitis Consortium. Notably, these protocols were developed using an integrative process based on prior consortium experience and with input from working groups with expertise in immunology, pancreatitis, and diabetes. Publication and adoption consistent biospecimen protocols will inform future studies and allow for better comparisons across different metabolic research efforts.

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