期刊
JOURNAL OF OLEO SCIENCE
卷 71, 期 10, 页码 1531-1540出版社
JAPAN OIL CHEMISTS SOC
DOI: 10.5650/jos.ess22207
关键词
colloids; antioxidants; vitamin E; radical scavenging; cytoprotection
资金
- University of Tokyo Advanced Characterization Nanotechnology Platform as a program ofNanotechnology Platformof the Ministry of Education, Culture, Sports, Science, and Technology(MEXT), Japan
- Tojyuro Iijima Foundation for Food Science and Technology
- Japan Society for the Promotion of Science [18K11001]
This study reveals that vitamin E nanoemulsion can be easily formed in water-based media through existing protocols. The nanosized vitamin E particles show excellent antioxidant performance and cell protection effect.
In molecular biology research, a vitamin E (VE) vehicle (VE dissolved in organic solvent) is often added to water media without a stabilizer. However, the detailed behavior of VE colloids in water media is unclear. In this study, we reveal that VE nanoemulsion readily forms in water-based media through the existing protocol. The colloid size was changed from 39 nm to the submicron scale by adjusting the initial concentration of the VE solution and adding a buffer. The radical scavenging effect of the dispersed nanosized VEs is comparable to that of the water-soluble antioxidant Trolox, providing excellent antioxidant performance in colloid form. The cytoprotection effect of the VE colloids under a lipid oxidation condition largely depends on the size of the nanodispersion. Smaller dispersed particles are more efficient radical scavengers than larger particles for a constant VE amount owing to sophisticated uptake behavior of cell. This unveiled fundamental knowledge pave the way for a preparative protocol of stabilizer-free VE vehicles, which are expected to become widely used in molecular biology research.
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