CD3+/CD56+ NKT-like Cells Show Imbalanced Control Immediately after Exercise in Delayed-Onset Muscle Soreness

The purpose of the study was to carry out an immunophenotypical characterization with a special focus on natural killer cells of junior swimmers from the Hungarian National Swim Team before and after an intensive acute exercise. Nineteen swimmers, ten females and nine males, completed the exercise protocol. Sixteen swimmers experienced delayed-onset muscle soreness. Most of our findings substantiated earlier results, such as the increase in the percentage of the CD3(-)/CD56(+) natural killer cells and the CD3(-)/CD56(dim+) NK cells, and the decrease in the percentage of CD3(+) T cells among lymphocytes after the exercise protocol. The drop of natural killer cell activity back to the pre-exercise level was in line with earlier findings. Interestingly, the percentage of CD3(+)/CD56(+) NKT-like cells did not change significantly in those three swimmers who did not report delayed-onset muscle soreness. On the contrary, the percentage of CD3(+)/CD56(+) NKT-like cells among lymphocytes increased in fourteen and decreased in two swimmers reporting delayed-onset muscle soreness. This study for the first time demonstrated a link between the delayed-onset muscle soreness and the imbalanced control of CD3(+)/CD56(+) NKT-like cells among lymphocytes. However, validation of this association in a larger sample size study will be necessary.

Automated summary

The purpose of this study was to investigate the immunophenotypical characterization, with a focus on natural killer cells, of junior swimmers from the Hungarian National Swim Team before and after intense exercise. The findings showed an increase in the percentage of CD3(-)/CD56(+) natural killer cells and CD3(-)/CD56(dim+) NK cells, as well as a decrease in the percentage of CD3(+) T cells among lymphocytes after exercise. Additionally, a link between delayed-onset muscle soreness and imbalanced control of CD3(+)/CD56(+) NKT-like cells among lymphocytes was demonstrated for the first time.