4.8 Article

Transcriptome-wide profiling of N6-methyladenosine via a selective chemical labeling method

期刊

CHEMICAL SCIENCE
卷 13, 期 41, 页码 12149-12157

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/d2sc03181g

关键词

-

资金

  1. Natural Science Foundation of China [91753201, 21721005]

向作者/读者索取更多资源

This study presents a chemical method (m(6)A-ORL-Seq) for high-throughput detection of m(6)A modifications in the transcriptome, and successfully identifies around four thousand high-confidence m(6)A sites in the human transcriptome. Unlike traditional antibody or enzyme-based methods, this new approach provides a novel perspective for single-base m(6)A detection at the transcriptome level.
Studies of chemical modifications on RNA have ushered in the field of epitranscriptomics. N-6-Methyladenosine (m(6)A) is the most typical RNA modification and is indispensable for basic biological processes. This study presents a chemical pulldown method (m(6)A-ORL-Seq) for transcriptome-wide profiling of m(6)A. Moreover, chemical labeling results in a specific reverse transcription (RT) truncation signature. This study has identified four thousand high-confidence m(6)A sites at single-base resolution in the human transcriptome. Unlike previously reported methods based on m(6)A-antibody or m(6)A-sensitive enzymes, the antibody/enzyme-free chemical method provides a new perspective for single-base m(6)A detection at the transcriptome level.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据