期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 116, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/54466
关键词
Biochemistry; Issue 116; Super-resolution imaging; PALM; Phosphatidylinositol 4; 5-bisphosphate (PI(4, 5) P2); cell membrane sheet; phosphoinositide; beta cells
资金
- National Institutes of Health (NIH) [R01DK093953, P30NS069271, BRFSG-2014-07]
- American Heart Associate pre-doctoral fellowship [14PRE20380168]
Phosphoinositides in the cell membrane are signaling lipids with multiple cellular functions. Phosphatidylinositol 4,5-bisphosphate (PI(4,5) P2) is a determinant phosphoinositide of the plasma membrane (PM), and it is required to modulate ion channels, actin dynamics, exocytosis, endocytosis, intracellular signaling, and many other cellular processes. However, the spatial organization of PI(4,5) P2 in the PM is controversial, and its nanoscale distribution is poorly understood due to the technical limitations of research approaches. Here by utilizing single molecule localization microscopy and the Pleckstrin Homology (PH) domain based dual color fluorescent probes, we describe a novel method to visualize the nanoscale distribution of PI(4,5) P2 in the PM in fixed membrane sheets as well as live cells.
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