期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 114, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/54098
关键词
Environmental Sciences; Issue 114; Sediment-water exchange; sediment biogeochemistry; denitrification; nitrogen cycling; sediment oxygen demand; benthic-pelagic coupling
资金
- Maryland Sea Grant Program
- National Science Foundation
- Maryland Sea Grant [R/AQ-5c, R/SV-2]
- NOAA Chesapeake Bay Office [NA13NMF4570210]
- Oyster Recovery Partnership
- National Science Foundation [OCE1427019]
- Exelon Corporation
- Maryland Environmental Service/Maryland Port Administration
- Directorate For Geosciences
- Division Of Ocean Sciences [1427019] Funding Source: National Science Foundation
The measurement of sediment-water exchange of gases and solutes in aquatic sediments provides data valuable for understanding the role of sediments in nutrient and gas cycles. After cores with intact sediment-water interfaces are collected, they are submerged in incubation tanks and kept under aerobic conditions at in situ temperatures. To initiate a time course of overlying water chemistry, cores are sealed without bubbles using a top cap with a suspended stirrer. Time courses of 4-7 sample points are used to determine the rate of sediment water exchange. Artificial illumination simulates day-time conditions for shallow photosynthetic sediments, and in conjunction with dark incubations can provide net exchanges on a daily basis. The net measurement of N-2 is made possible by sampling a time course of dissolved gas concentrations, with high precision mass spectrometric analysis of N-2:Ar ratios providing a means to measure N-2 concentrations. We have successfully applied this approach to lakes, reservoirs, estuaries, wetlands and storm water ponds, and with care, this approach provides valuable information on biogeochemical balances in aquatic ecosystems.
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