4.4 Article

Measuring the linear viscoelastic regime of MCF-7 cells with a monolayer rheometer in the presence of microtubule-active anti-cancer drugs at high concentrations

期刊

INTERFACE FOCUS
卷 12, 期 6, 页码 -

出版社

ROYAL SOC
DOI: 10.1098/rsfs.2022.0036

关键词

anti-cancer drugs; cell monolayer rheology; linear viscoelasticity; microtubules; narrow-gap rheometry

类别

资金

  1. National Research Foundation of Korea (NRF) - Korean government (MSIT) [NRF-2020R1A2C2014792]

向作者/读者索取更多资源

The rheological properties of cells have functional implications and can be analyzed using microfluidic devices and rheometers. This technique allows for the fast analysis and study of cell deformation and vitality. However, the impact of experimental conditions on the results should be considered when studying the effects of microtubule-active drugs and cells with size variation.
The rheological properties of cells have vital functional implications. Depending, for instance, on the life cycle, cells show large cell-to-cell variations making it cumbersome to quantify average viscoelastic properties of cells by single-cell techniques. Microfluidic devices, typically working in the nonlinear viscoelastic range, allow fast analysis of single-cell deformation. Averaging over a large number of cells can also be achieved by studying them in a monolayer between rheometer discs. This technique allows applying well-established rheological standard procedures to cell rheology. It offers further advantages like studying cells in the linear viscoelastic range while quantifying cell vitality. Here, we study the applicability of the technique to rather adverse conditions, like for microtubule-active anti-cancer drugs and for a cell line with large size variation. We found a strong impact of the gap width and of normal forces on the moduli and obtained high vitality levels during the rheological study. To enable studying the impact of microtubule-active drugs on vital cells at concentrations several orders of magnitude beyond the half maximal effective concentration for cytotoxicity, we arrested the cell cycle with hydroxyurea. Irrespective of the high concentrations, we observed no clear impact of the microtubule-active drugs.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据