A novel biodegradable polymer scaffold for in vitro growth of corneal epithelial cells

The shortage of donor corneal tissue worldwide has led to extensive research for alternate corneal equivalents utilizing tissue engineering methods. We conducted experiments using Poly D, L lactic acid polymer along with a copolymer (Eudragit) in varying concentrations to create a biodegradable scaffold suitable for in vitro growth of corneal epithelial stem cells. It was found that stable, spherical, and porous microparticles can be prepared by combining PDLLA and Eudragit RL100 polymers in the ratio of 90:10 and 70:30. The microparticles can then be fused to form scaffold membranes with porous architecture and good water retention capacity at room temperature using methanol, which can withstand handling during transplantation procedures. The scaffolds made using a 70:30 ratio were found to be suitable for the promotion of growth of laboratory corneal epithelial stem cell lines (SIRC cell lines). This innovation can pave way for further developments in corneal stem cell research and growth, thus providing for viable laboratory-derived corneal substitutes.

Automated summary

The shortage of donor corneal tissue has prompted research on tissue engineering methods to create alternatives. In this study, a biodegradable scaffold was developed using a mixture of PDLLA and Eudragit polymers. The scaffold showed stability, porosity, and water retention capacity, making it suitable for in vitro growth of corneal epithelial stem cells. The scaffold made with a 70:30 ratio of PDLLA to Eudragit was found to promote the growth of laboratory corneal epithelial stem cells.