4.7 Article

Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells

期刊

GELS
卷 8, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/gels8100680

关键词

mesenchymal stem cells; osteoblastogenesis; piezoelectricity; poly(vinylidene) fluoride; hydrogel

资金

  1. Spanish State Research Agency (AEI)
  2. VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program
  3. Instituto de Salud Carlos III
  4. European Regional Development
  5. FCT (Fundacao para a Ciencia e a Tecnologia) [UID/FIS/04650/2021, PTDC/FIS-MAC/28157/2017, POCI-01-0145-FEDER-007688, 2020.04028 CEECIND]
  6. [PID2019-106000RB-C21/AEI/10.13039/501100011033]
  7. [PID2019-106099RB-C41]
  8. [PID2019-106099RB-C43/AEI/10.13039/501100011033]
  9. [BES-2017-080398]

向作者/读者索取更多资源

Piezoelectric stimulation promotes pre-differentiation of MSCs, and PVDF-CFO cell culture supports offer a wireless stimulation strategy. MSCs embedded in electroactive microspheres can be locally stimulated. This magnetically activated 3D electroactive cell culture support can be used for pre-differentiation of MSCs before transplantation.
Mesenchymal stem cells (MSCs) osteogenic commitment before injection enhances bone regeneration therapy results. Piezoelectric stimulation may be an effective cue to promote MSCs pre-differentiation, and poly(vinylidene) fluoride (PVDF) cell culture supports, when combined with CoFe2O4 (CFO), offer a wireless in vitro stimulation strategy. Under an external magnetic field, CFO shift and magnetostriction deform the polymer matrix varying the polymer surface charge due to the piezoelectric effect. To test the effect of piezoelectric stimulation on MSCs, our approach is based on a gelatin hydrogel with embedded MSCs and PVDF-CFO electroactive microspheres. Microspheres were produced by electrospray technique, favouring CFO incorporation, crystallisation in beta-phase (85%) and a crystallinity degree of around 55%. The absence of cytotoxicity of the 3D construct was confirmed 24 h after cell encapsulation. Cells were viable, evenly distributed in the hydrogel matrix and surrounded by microspheres, allowing local stimulation. Hydrogels were stimulated using a magnetic bioreactor, and no significant changes were observed in MSCs proliferation in the short or long term. Nevertheless, piezoelectric stimulation upregulated RUNX2 expression after 7 days, indicating the activation of the osteogenic differentiation pathway. These results open the door for optimising a stimulation protocol allowing the application of the magnetically activated 3D electroactive cell culture support for MSCs pre-differentiation before transplantation.

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