HPV16 infection in oral cavity and oropharyngeal cancer patients

Our objective was to compare archived tissue biopsy samples from the oral cavity and oropharynx, in terms of human papillomavirus (HPV) 16 infection. We used Taqman real-time PCR assay to detect HPV16 in 121 archived biopsy samples from the oral cavity and 100 samples from the oropharynx. Among patients with oral cavity cancer (OCC), 9% (6/65) had HPV16 infection which was significantly less than those with oropharyngeal cancer (OPC) where 79% (39/50) were HPV16 positive (P < 0.001). Our results demonstrated a significant difference between genders where males had a seven times higher chance of having HPV16 infection (P < 0.001). Viral load variation between each group was demonstrated. The median viral load in OPC was similar in OCC cases, but cancer samples were significantly higher than in non-cancer cases (oral cavity samples P = 0.015; oropharynx samples P = 0.09). From our results, we conclude that there is a significant difference in HPV16 detection between OCC and OPC, and HPV16 differs greatly between male and female cancer patients.