期刊
HORTICULTURAE
卷 8, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/horticulturae8100891
关键词
Cucumis melo; waterlogging; transcriptome; molecular response
类别
资金
- Natural Science Foundation of China [32260756]
- China Postdoctoral Science Foundation [2021M701514]
- Modern Agricultural Research Collaborative Innovation Program of Jiangxi Province [JXXTCXQN202007, JXXTCX202109, JXXTCX202203]
- Innovation Program of Jiangxi Academy of Agricultural Sciences [20181CBS002]
- China Agriculture Research System [CARS-25]
- Jiangxi Province Science Foundation for Youths [20192BAB214017]
- Agricultural Science and Technology Innovation Program [CAAS-ASTIP-2021-ZFRI]
This study investigated the physiological and transcriptomic responses to waterlogging stress in two melon accessions ('L39' and 'L45'). The results showed that 'L45' exhibited higher chlorophyll content and lower electrolyte leakage and malondialdehyde content compared to 'L39' under waterlogging stress. Gene expression analysis revealed that genes related to chlorophyll synthesis and photosynthesis were more suppressed in 'L39', while genes related to sugar cleavage, glycolysis, and fermentation were highly induced. Differentially expressed transcription factors, particularly members of the ERF, bHLH, and WRKY families, were identified as potentially important for waterlogging tolerance in melon.
Waterlogging is a serious abiotic stressor that drastically hinders the growth and productivity of melon (Cucumis melo) around the world, due to the reduction in available oxygen levels in the waterlogged tissues. However, the mechanism underlying the responses to waterlogging stress in melon is largely unknown. In this study, physiological and transcriptome data of the waterlogging-sensitive accession 'L39' and the waterlogging-tolerant accession 'L45' were investigated under conditions of normal water supply and waterlogging stress. The results showed that 'L45' exhibited higher chlorophyll contents and lower REL (relative electrolyte leakage) and MDA (malondialdehyde) contents compared with 'L39' under waterlogging stress. Additionally, waterlogging stress only led to the stomatal closure and chloroplast damage of 'L39'. In total, 1748 genes were differentially expressed in the leaves of waterlogging-stressed 'L45' compared with control, whereas 3178 genes were differentially expressed in 'L39'. Further analysis indicated that genes related to chlorophyll synthesis and photosynthesis were more depressed in 'L39', while sugar cleavage, glycolysis, and fermentation genes were highly induced in 'L39' compared with 'L45'. The expression of genes involved in ROS (reactive oxygen species) scavenging and hormone signaling significantly differed between 'L39' and 'L45' in their response to waterlogging stress. Moreover, a total of 311 differentially expressed transcription factors were waterlogging-responsive, among which members of the ERF (ethylene response factor), bHLH (basic helix-loop-helix), and WRKY families might play crucial roles in waterlogging tolerance in melon. This study unraveled the molecular responses to waterlogging stress in melon and could provide helpful candidate genes for further molecular breeding of waterlogging-tolerant melon varieties.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据