期刊
GELS
卷 8, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/gels8100611
关键词
adipose tissue; 3D bioprinting; hydrogels; primary human adipose-derived stem cells; primary human mature adipocytes; gelatin methacryloyl
资金
- European Commission
- Federal Ministry of Education and Research [1099895]
- Ministry of Science, Research, and Arts Baden Wurttemberg [IP2019]
This study investigates the relationship between adipose tissue and multiple diseases, highlighting the importance of suitable in vitro models for research purposes. The researchers successfully developed physiological in vitro adipose tissue models using bioprinting technology.
Adipose tissue is related to the development and manifestation of multiple diseases, demonstrating the importance of suitable in vitro models for research purposes. In this study, adipose tissue lobuli were explanted, cultured, and used as an adipose tissue control to evaluate in vitro generated adipose tissue models. During culture, lobule exhibited a stable weight, lactate dehydrogenase, and glycerol release over 15 days. For building up in vitro adipose tissue models, we adapted the biomaterial gelatin methacryloyl (GelMA) composition and handling to homogeneously mix and bioprint human primary mature adipocytes (MA) and adipose-derived stem cells (ASCs), respectively. Accelerated cooling of the bioink turned out to be essential for the homogeneous distribution of lipid-filled MAs in the hydrogel. Last, we compared manual and bioprinted GelMA hydrogels with MA or ASCs and the explanted lobules to evaluate the impact of the printing process and rate the models concerning the physiological reference. The viability analyses demonstrated no significant difference between the groups due to additive manufacturing. The staining of intracellular lipids and perilipin A suggest that GelMA is well suited for ASCs and MA. Therefore, we successfully constructed physiological in vitro models by bioprinting MA-containing GelMA bioinks.
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