4.7 Article

Secretion of Bacillus amyloliquefaciens Transglutaminase from Lactococcus lactis and Its Enhancement of Food Gel Properties

期刊

GELS
卷 8, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/gels8100674

关键词

Lactococcus lactis; microbial transglutaminase (MTGase); SPI gel

资金

  1. Major Project of Science and Technology of Anhui Province [201903a06020007, 202103b06020009, 202003b06020017]
  2. Natural Science Foundation of Anhui Province [2108085MC123]
  3. Fundamental Research Funds for the Central Universities [PA2021KCPY0048]
  4. China Postdoctoral Science Foundation [2019M651013]

向作者/读者索取更多资源

This study successfully heterologously expressed BaMTGase in Lactococcus lactis and purified it by directing secretion using a signal peptide. BaMTGase showed superior performance in SPI solution crosslinking, with higher gel strength and water-holding capacity. This work provides an attractive cell factory for the food industry and a suitable platform for MTGase production.
(1) Background: Microbial transglutaminases (MTGase) catalyze protein crosslink. This is useful in the food industry to improve gelation, water holding capacity, and emulsifying capacity during foodstuff manufacturing. The production of MTGase in wild-type strains renders low yield and high costs of downstream purification, limiting its industrial applications. (2) Methods: In this work, MTGase from Bacillus amyloliquefaciens BH072 (BaMTGase) has been heterologously expressed in Lactococcus lactis, using the signal peptide Usp45 to direct the secretion of recombinant BaMTGase out of the cell for easier purification. (3) Results: In these conditions, MTGase was purified with a high yield (48.7 +/- 0.2 mg/L) and high enzyme activity (28.6 +/- 0.5 U/mg). Next, BaMTGase was tested for industrial applications. Recombinant BaMTGase and commercial MTGase were used for SPI solution crosslinking. BaMTGase formed a harder gel with higher water-holding capacity and a dense and smooth gel microstructure. (4) Conclusions: This work provides an attractive food-grade cell factory for the food industry and offers a suitable chassis for MTGase production.

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