期刊
VETERINARY RESEARCH
卷 53, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13567-022-01110-4
关键词
Heat-labile enterotoxin; ETEC; adherence; fimbriae; pathogenesis
资金
- Chinese National Science Foundation [31800121]
- Jiangsu Science and Technology Bureau Project [BE2022348]
- Jiangsu Joint Laboratory for International Cooperation in Prevention and Control of Important Animal Infectious Diseases and Zoonoses
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Heat-labile enterotoxin enhances bacterial adherence by upregulating adhesin and enterotoxin expression in enterotoxigenic Escherichia coli. These findings provide insights into the pathogenic mechanism of heat-labile enterotoxin and enterotoxigenic Escherichia coli.
As one of the crucial enterotoxins secreted by enterotoxigenic Escherichia coli (ETEC), heat-labile enterotoxin (LT) enhances bacterial adherence both in vivo and in vitro; however, the underlying mechanism remains unclear. To address this, we evaluated the adherence of LT-producing and LT-deficient ETEC strains using the IPEC-J2 cell model. The expression levels of inflammatory cytokines and chemokines, and tight-junction proteins were evaluated in IPEC-J2 cells after infection with various ETEC strains. Further, the levels of adhesins and enterotoxins were also evaluated in F4ac-producing ETEC (F4 + ETEC) strains after treatment with cyclic AMP (cAMP). The adherence of the Delta eltAB mutant was decreased compared with the wild-type strain, whereas adherence of the 1836-2/pBR322-eltAB strain was markedly increased compared with the 1836-2 parental strain. Production of LT up-regulated the expression of TNF-alpha, IL-6, CXCL-8, and IL-10 genes. However, it did not appear to affect tight junction protein expression. Importantly, we found that cAMP leads to the upregulation of adhesin production and STb enterotoxin. Moreover, the F4 + ETEC strains treated with cAMP also had greater adhesion to IPEC-J2 cells, and the adherence of Delta faeG, Delta fliC, and Delta estB mutants was decreased. These results indicate that LT enhances the adherence of F4 + ETEC due primarily to the upregulation of F4 fimbriae, flagellin, and STb enterotoxin expression and provide insights into the pathogenic mechanism of LT and ETEC.
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