Hierarchically mesoporous Ce-based MOFs with enhanced alkaline phosphatase-like activity for phosphorylated biomarker sensing

Herein, we develop a hierarchically mesoporous cerium metal-organic framework (Ce-HMMOF) nanozyme with enhanced ALP-mimicking activity for the naked-eye detection of phosphorylated biomarkers. The long-range ordered mesochannels (9.18 nm) throughout the Ce-HMMOF promote both the mass transfer and the accessibility of interior active sites, permitting the rapid and sensitive sensing of phosphorylated biomarkers through ALP-like biocatalysis. This work provides a new insight into the engineering of highly active nanozymes for disease-associated biomarker screening and diagnosis.

Automated summary

A hierarchically mesoporous cerium metal-organic framework (Ce-HMMOF) nanozyme with enhanced ALP-mimicking activity is developed for naked-eye detection of phosphorylated biomarkers. The long-range ordered mesochannels in Ce-HMMOF promote mass transfer and accessibility of interior active sites, enabling rapid and sensitive sensing of phosphorylated biomarkers through ALP-like biocatalysis. This work provides new insights into the engineering of highly active nanozymes for disease-associated biomarker screening and diagnosis.