期刊
ARTHRITIS RESEARCH & THERAPY
卷 24, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13075-022-02938-z
关键词
Behcet's disease; Macrophage polarization; Phagocytosis; Th1 differentiation; NF-kappa B pathway
类别
资金
- National Natural Science Foundation of China [81571598, 81871299, 82171800]
- National High Level Hospital Clinical Research Funding [2022-PUMCH-C-008]
- CAMS Innovation Fund for Medical Sciences [2022-I2M-CT-B-006]
This study investigated the effect of BD serum on the phenotypes and functions of macrophage polarization. The findings revealed that BD serum promoted macrophage polarization towards a proinflammatory M1-like phenotype through NF-kappa B signaling. This may contribute to inflammation in BD. The study suggests that M1 polarized macrophages could be a potential therapeutic target for BD.
Background: Macrophages are key innate immune cells implicated in the pathogenesis of Behcet's disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization. Methods: BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4(+)T cell differentiation facilitated by HMDMs were measured by flow cytometry. Transcriptome analysis of BD and HC serum-stimulated HMDMs was conducted to identify differentially expressed genes. NF-kappa B signaling was examined using western blot to explore the mechanism of macrophage polarization induced by BD serum. Results: BD serum-treated macrophages expressed a higher level of CD86, IL-12, and TNF-alpha and a lower level of CD163, which were compatible with the M1-like phenotype. Furthermore, BD serum-treated macrophages showed enhanced phagocytic capacity and promoted more Th1 cell differentiation. Sixty-one differentially expressed genes were identified between BD and HC serum-treated macrophages and were enriched in NF-kappa B signaling. BD serum-treated macrophages showed upregulated p-p65 and downregulated IKB alpha, and NF-kappa B inhibitor attenuated BD serum-stimulated M1-like phenotype. Conclusions: BD serum promoted macrophage polarization toward a proinflammatory M1-like phenotype through NF-kappa B signaling and potentially facilitated inflammation in BD. M1 polarized macrophages may be a potential therapeutic target for BD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据