期刊
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
卷 7, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s40104-016-0063-2
关键词
Intercellular junctions; Kv channels; Small intestine; Piglets; Weaning
资金
- National Key Basic Research Program of China [2013CB127302]
- National Natural Science Foundation of China [31330075, 31372326, 31301988, 31301989]
- State Key Laboratory of Animal Nutrition [2004DA125184F1401]
- Spark Program of Jiangxi Province [20142BBF061051]
- Changsha Lvye Biotechnology Limited Company Academician Expert Workstation
- Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine
- Guangdong Hinapharm Group Academician Workstation for Biological Feed and Feed Additives
- Animal Intestinal Health
Background: The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different intercellular junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, intercellular junctions and voltage-gated potassium (Kv) channels in the intestine of piglets during the suckling and post-weaning periods. Results: Samples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen (PCNA)-positive cells and alkaline phosphatase (AKP) activity, as well as the abundances of E-cadherin, occludin, and Kv1.5 mRNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1 to d 21 during the suckling period (P < 0.05). Weaning induced decreases in the percentage of PCNA-positive cells, AKP activity and the abundances of E-cadherin, occludin and zonula occludens (ZO)-1 mRNA or protein in the jejunum on d 1, 3 and 5 post-weaning (P < 0.05). There were lower abundances of E-cadherin, occludin and ZO-1 mRNA as well as claudin-1, claudin-3 and ZO-1 protein in the jejunum of weanling piglets than in 21-d-old suckling piglets (P < 0.05). The abundances of E-cadherin, occludin, ZO-1 and integrin mRNA were positively related to the percentage of PCNA-positive cells. Conclusion: Weaning at 14 d of age induced damage to the intestinal morphology and barrier. While there was an adaptive restoration on d 7 post-weaning, the measured values did not return to the pre-weaning levels, which reflected the impairment of intercellular junctions and Kv channels.
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