☆ 4.6 Review

Advances in Komagataella phaffii Engineering for the Production of Renewable Chemicals and Proteins

FERMENTATION-BASEL (2022)

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Article Biotechnology & Applied Microbiology

Adaptive laboratory evolution and reverse engineering enhances autotrophic growth in Pichia pastoris

Thomas Gassler et al.

Summary: Synthetic biology offers multiple pathways for converting CO2 into biomass or bio-chemicals, reducing the unsustainable use of organic feedstocks that contribute to climate change. The use of industrial organisms like Pichia pastoris allows for the development of novel bioproduction platforms with reduced reliance on alternative food production resources. By rewiring the central carbon metabolism and utilizing reverse genetic engineering, it is possible to enhance the autotrophic growth of microbes and explore potential applications for producing value-added products using CO2.

METABOLIC ENGINEERING (2022)

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Article Chemistry, Multidisciplinary

Methylotrophy of Pichia pastoris: Current Advances, Applications, and Future Perspectives for Methanol-Based Biomanufacturing

Yuanke Guo et al.

Summary: Methanol, a potential substitute for sugar-based raw materials in biomanufacturing, has gained significant attention. This review focuses on the application of Pichia pastoris, a methylotrophic cell factory, in methanol-based biomanufacturing. The article discusses the current understanding of methanol metabolism in P. pastoris, important factors influencing its methanol utilization ability, recent advances in using engineered P. pastoris for producing various chemicals from methanol, as well as future challenges and potential solutions in developing P. pastoris as an efficient cell factory for methanol-based biomanufacturing.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2022)

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Review Biochemistry & Molecular Biology

Production of myo-inositol: Recent advance and prospective

Yunjie Li et al.

Summary: The paper provides a comprehensive overview of myo-inositol production methods, including chemical acid hydrolysis, microbial fermentation, and in vitro enzymatic biocatalysis. It highlights the environmental issues associated with chemical hydrolysis and presents innovative strategies for efficient myo-inositol biosynthesis through microbial fermentation. In vitro enzymatic biocatalysis is identified as a promising strategy for future myo-inositol industrial biomanufacturing due to its low cost and high productivity.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2022)

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Article Biotechnology & Applied Microbiology

Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production

Arnau Gasset et al.

Summary: The combination of strain and bioprocess engineering strategies was investigated to improve recombinant protein production (RPP) in the yeast P. pastoris. By comparing different gene dosage and oxygen-limiting conditions, higher specific production rate (q(P)) values were achieved. Additionally, applying hypoxia in carbon-limited fed-batch cultures further increased q(P). Transcriptional analysis indicated potential limitations in protein processing and transcription attenuation in high gene dosage strains. This study demonstrates the importance of strain and bioprocess engineering in optimizing RPP and developing the bioeconomy.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

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Article Biotechnology & Applied Microbiology

Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization

Lina Heistinger et al.

Summary: By characterizing 25 natural isolates from all seven described Komagataella species, this study identified strains with differences in tolerance to high pH, high temperature, and growth on xylose. The genotypic and phenotypic diversity within this genus provides a basis for utilizing neglected Komagataella strains with interesting characteristics and understanding genetic determinants for improved growth and stress tolerance.

MICROBIAL CELL FACTORIES (2022)

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Article Biochemistry & Molecular Biology

Metabolic engineering of Komagataella phaffii for synergetic utilization of glucose and glycerol

Xiaolu Wang et al.

Summary: This study addresses the challenge of carbon flux distribution in K. phaffii by developing a strategy for the synergetic utilization of glucose and glycerol. The findings provide valuable insights for engineering K. phaffii as a versatile fermentation platform for biochemical production.

YEAST (2022)

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Article Biochemical Research Methods

Construction of an L-Tyrosine Chassis in Pichia pastoris Enhances Aromatic Secondary Metabolite Production from Glycerol

Ryota Kumokita et al.

Summary: This study aimed to construct a high-yield L-Tyr microbial chassis in the unconventional yeast P. pastoris and enhance the production of various aromatic compounds through heterologous pathway expression. The engineered P. pastoris strains showed significantly increased L-Tyr production from glycerol and efficiently produced resveratrol, naringenin, and norcoclaurine. Additionally, crude glycerol byproducts from biodiesel fuel production were utilized for the production of L-Tyr-derived aromatic compounds.

ACS SYNTHETIC BIOLOGY (2022)

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Review Biotechnology & Applied Microbiology

Comprehensive review on biotechnological production of hyaluronic acid: status, innovation, market and applications

Ruschoni Ucm et al.

Summary: The growing demand for low-cost and high-quality hyaluronic acid (HA) can be met by producing HA from harmless microorganisms, leading to economic and environmentally friendly production. Various fermentation modes and downstream processing techniques have been explored to optimize HA production at different molecular weights.

BIOENGINEERED (2022)

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Review Biotechnology & Applied Microbiology

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Congqiang Zhang et al.

Summary: The global shift towards sustainability has led to the exploration of alternative feedstocks for biomanufacturing. C1 and C2 substrates, such as CO2, CO, methane, formate, methanol, acetate, and ethanol, are gaining attention due to their natural abundance and low production cost. Advances in metabolic engineering and synthetic biology have improved the efficiency of using these feedstocks by microbes, which can be further engineered to convert them into valuable products.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

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Review Biochemistry & Molecular Biology

Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

Cristina Bustos et al.

Summary: Komagataella phaffii has become an important microorganism for recombinant protein production, particularly in industrial settings. Significant progress has been made in improving recombinant protein productivity and reducing production costs. Cell engineering approaches, including metabolic engineering, transcription factor modulation, and manipulation of protein folding and secretion pathways, have shown promising results. However, more research is needed in optimizing cultivation parameters in larger-scale bioreactors.

METABOLITES (2022)

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Article Biotechnology & Applied Microbiology

Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism

Qiquan Zhang et al.

Summary: This study successfully engineered P. pastoris for efficient inositol production through a dynamic regulation strategy, achieving a high inositol titer and providing valuable guidance for producing other high-value bioproducts using this yeast.

MICROBIAL CELL FACTORIES (2022)

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Article Engineering, Multidisciplinary

A multi-model predictive control method for the Pichia pastoris fermentation process based on relative error weighting algorithm

Bo Wang et al.

Alexandria Engineering Journal (2022)

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Review Biotechnology & Applied Microbiology

Recent progress in the microbial production of xylonic acid

Debora Trichez et al.

Summary: This article summarizes the recent advances in producing xylonic acid from xylose oxidation. Although industrial-scale production of xylonic acid has not yet been achieved, several microbial bio-based production processes are being developed. The article highlights pathway characterization, genetic engineering, and fermentative strategies to improve xylonic acid production, as well as the major requirements for achieving commercial-scale production.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2022)

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Review Biotechnology & Applied Microbiology

From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains

Camille Peiro et al.

Summary: Engineering microorganisms to grow on alternative feedstocks is crucial for biotechnological applications and advancing our understanding of microbial metabolism. Synthetic methylotrophy, which involves completely changing a microorganism's lifestyle and utilizing methanol as a sole carbon source, is the pinnacle of metabolic engineering. Recent advances in optimizing host metabolism, mixing and matching enzymes, and creating novel enzymatic functions have propelled the field of synthetic methylotrophy further than ever before.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

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Article Chemistry, Multidisciplinary

Synthesis of bioengineered heparin by recombinant yeast Pichia pastoris

Yonglin Zhang et al.

Summary: The bioengineered heparin synthesized using a yeast platform, with a conversion ratio of 41% from heparosan, exhibits comparable anticoagulant activity to commercial heparin from animals. This innovative approach has the potential to produce non-animal sourced heparin, presenting promising prospects in the field of anticoagulant drug development.

GREEN CHEMISTRY (2022)

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Article Biotechnology & Applied Microbiology

Screening method to prioritize relevant bio-based acids and their biochemical processes using recent patent information

Melissa Braga et al.

Summary: This study analyzes scientific papers and patents to identify the acids in the bio-based carboxylic acid field that are generating growing scientific and industrial interest. The results show that acids of substantial commercial interest, such as acetic, citric, lactic, acrylic, and methacrylic acids, have the highest growth rate both in scientific papers and patents. The analysis also reveals that the most relevant processes in patents are associated with 'drop-in' acids such as methacrylic, adipic, maleic, acrylic, and glutaric acids.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR (2021)

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Article Biotechnology & Applied Microbiology

Metabolic engineering ofPichia pastorisfor malic acid production from methanol

Feng Guo et al.

Summary: The study demonstrates the application of rational design and gene regulation in engineeringP. pastoristo produce malic acid from sole methanol, achieving a significant increase in malic acid yield through optimization of accumulation modules and gene knockouts. The results provide important guidance and reference for metabolic engineering ofP. pastoristo produce value-added chemicals.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

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Review Biology

Bioprocess Control: Current Progress and Future Perspectives

Anurag S. Rathore et al.

Summary: Bioprocess control involves unique challenges such as non-linearity, variability, and complexity, requiring the use of modern control strategies. In addition to automation, control also includes aspects such as system architecture, software applications, hardware, and interfaces, all of which need to be optimized according to actual needs.

LIFE-BASEL (2021)

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Article Biotechnology & Applied Microbiology

Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium

Moo Sun Hong et al.

Summary: This article constructs a macroscopic bioreactor model for Pichia pastoris to describe cellular metabolism and optimize bioreactor operation. The model predictions accurately describe experimental data and can be used to improve chemically defined medium.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

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Article Biochemistry & Molecular Biology

Application of Rhizomucor miehei lipase-displaying Pichia pastoris whole cell for biodiesel production using agro-industrial residuals as substrate

Raphael Oliveira Sena et al.

Summary: This study successfully utilized a novel biocatalyst RML-PIR1 for producing biodiesel from residual agro-industrial fatty acids. The biocatalyst demonstrated optimal activity at 45 degrees C, with high stability and enhanced reuse potential through treatment with GA and PEG. This new biocatalyst shows promising potential for biotechnological applications, particularly in large-scale biofuel production.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

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Review Microbiology

Komagataella phaffii as Emerging Model Organism in Fundamental Research

Lukas Bernauer et al.

Summary: K. phaffii is an important model organism for studying eukaryotic molecular cell biology, with the intriguing property of being able to efficiently assimilate methanol. Research topics on this yeast range from studying methanol assimilation, peroxisome biogenesis and pexophagy to other cellular processes such as protein secretion, lipid biosynthesis and cell wall biogenesis. Comparing data obtained from K. phaffii with other yeasts can elucidate major differences and highlight its big potential in fundamental research.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Green & Sustainable Science & Technology

Development of the Circular Bioeconomy: Drivers and Indicators

Maximilian Kardung et al.

Summary: The study proposes a conceptual framework for quantifying and analyzing the development of the EU bioeconomy, focusing on the synergies between the bioeconomy and circular economy, measurement of bio-based sectors, and indicators aligned with the objectives of the EU's bioeconomy strategy.

SUSTAINABILITY (2021)

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Article Biotechnology & Applied Microbiology

Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors

Barbara G. Paes et al.

Summary: The study demonstrated for the first time the dose-dependent response of P. pastoris to acetic acid, furaldehydes, and sugarcane biomass hydrolysate. It was found that the yeast could grow at certain concentrations, but was completely inhibited at high concentrations of hydrolysate. Transcriptomic analysis revealed a polygenetic response in P. pastoris to lignocellulose-derived inhibitors involving up-regulation and repression of various genes.

AMB EXPRESS (2021)

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Review Biotechnology & Applied Microbiology

Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts

Pradipta Patra et al.

Summary: Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon sources is a growing area in industrial research. Non-conventional yeasts can be selected as hosts to avoid extensive strain engineering of model yeast Saccharomyces cerevisiae for biosynthesis of commercial molecules other than ethanol. These non-conventional yeasts offer desirable phenotypes for industrial usage, but advanced metabolic engineering efforts in them are still lacking due to limited availability of systems and synthetic biology methods.

BIOTECHNOLOGY ADVANCES (2021)

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Article Biotechnology & Applied Microbiology

Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor

Tai Man Louie et al.

Summary: The study introduced a two-stage strategy for producing bio-xylitol from D-xylose using recombinant Pichia pastoris expressing a heterologous xylose reductase gene. The biotransformation was shown to be efficient with high conversion rates, and the whole cells could be reused without loss of activity. This method can be applied to other oxidoreductase reactions, demonstrating its potential for various industrial applications beyond xylitol production.

MICROBIAL CELL FACTORIES (2021)

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Article Biochemistry & Molecular Biology

Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects

Zeynep Efsun Duman-Ozdamar et al.

Summary: Industrial enzymes are widely used in various industries due to their economic and environmental benefits. P. pastoris has shown great potential as a host for heterologous protein expression, especially in high-cell density fermentation. Advances in gene-editing tools have expanded the possibilities for improving strains of P. pastoris, while research is focused on enhancing recombinant protein production methods.

PROTEIN JOURNAL (2021)

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Review Multidisciplinary Sciences

Applications, challenges, and needs for employing synthetic biology beyond the lab

Sierra M. Brooks et al.

Summary: Current developments in synthetic biology are not immediately applicable outside of the controlled laboratory environment. In this Perspective, the authors outline the advances and challenges the field faces in operating in resource limited and off-the-grid scenarios.

NATURE COMMUNICATIONS (2021)

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Review Biotechnology & Applied Microbiology

Waste biorefinery towards a sustainable circular bioeconomy: a solution to global issues

Hui Yi Leong et al.

Summary: Circular bioeconomy is a promising approach for addressing global issues such as environmental problems and food security by utilizing bioenergy and biomaterials production to create a cleaner and low carbon environment. Waste biorefinery as a sustainable bio-based circular economy contributes to a greener environment by producing useful products and metabolites from waste materials. Integrating waste biorefinery with circular bioeconomy strategies can help tackle carbon management and greenhouse gas emissions, leading towards a sustainable and greener world.

BIOTECHNOLOGY FOR BIOFUELS (2021)

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Review Biochemistry & Molecular Biology

Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris

Sonakshi De et al.

Summary: Pichia pastoris has become the most popular yeast cell factory for producing heterologous proteins, with strong promoters, stable genetic constructs, and a growing collection of strains, tools, and protocols. In addition to small-scale production for biochemical studies and protein characterization, the yeast is also being increasingly used for in vivo biotransformation and metabolic pathway engineering for chemical production.

MICROBIAL CELL FACTORIES-BOOK (2021)

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Review Biotechnology & Applied Microbiology

Microbial engineering for the production of isobutanol: current status and future directions

Nair M. Lakshmi et al.

Summary: Isobutanol has gained attention as an alternate fuel due to its minimal effects on the atmosphere and its various industrial applications. Genetic and metabolic engineering approaches have been successful in increasing isobutanol production in microbial strains, allowing for more efficient and sustainable production. Fermentation using cost-effective lignocellulosic biomass and an efficient downstream process can yield isobutanol in an environmentally friendly manner.

BIOENGINEERED (2021)

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Article Biotechnology & Applied Microbiology

Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9

Javier Garrigos-Martinez et al.

Summary: The study presents a scalable bioprocess for fine-tuned co-expression of hCYP2C9 and hCPR in yeast Pichia pastoris. High-throughput screening was used to optimize co-expression and identify best expressing variants. Rational optimization criteria were applied for developing a bioprocess in bioreactors, leading to significantly increased production rates and yield.

MICROBIAL CELL FACTORIES (2021)

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Review Green & Sustainable Science & Technology

Conversion of lignocellulose to biofuels and chemicals via sugar platform: An updated review on chemistry and mechanisms of acid hydrolysis of lignocellulose

Ziyuan Zhou et al.

Summary: This paper reviews the acid hydrolysis of lignocellulose for sugar release, focusing on the chemistry and kinetics of concentrated acid and dilute acid hydrolysis. Challenges such as the difficulty in separating acid and sugar, high temperature requirements, and sugar degradation are discussed. Strategies for efficient recovery of acid catalysts, increasing sugar yield, and reducing sugar degradation need to be developed to overcome obstacles for commercialization. Integration and kinetic control of the process could help reduce energy consumption and extra cost of post-treatment. Solid acid hydrolysis, while advantageous for catalyst reuse, still has a long way to go for practical application.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2021)

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Article Chemistry, Multidisciplinary

Biovalorization of Lignocellulosic Materials for Xylitol Production by the Yeast Komagataella pastoris

Diana Araujo et al.

Summary: This study screened different lignocellulosic materials for supporting yeast cell growth and xylitol production, finding banana peels and grape stalks to be the most effective for growth, and brewer's spent grains for xylitol production. Detoxification with activated charcoal significantly improved cell growth and xylitol production from brewer's spent grains.

APPLIED SCIENCES-BASEL (2021)

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Article Biotechnology & Applied Microbiology

Benchmarking recombinant Pichia pastoris for 3-hydroxypropionic acid production from glycerol

Albert Fina et al.

Summary: This study demonstrated the use of P. pastoris as a cell factory to produce 3-hydroxypropionic acid from glycerol. By introducing the mcr gene from Chloroflexus aurantiacus and optimizing enzyme and metabolic engineering modifications, a significant increase in 3-HP production was achieved. The best strain (PpHP6) showed a high volumetric productivity, indicating the promising potential of P. pastoris for the production of bulk chemicals, especially 3-HP, from crude glycerol.

MICROBIAL BIOTECHNOLOGY (2021)

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Article Biotechnology & Applied Microbiology

Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan

Ting Miao et al.

Summary: The study improved the production of Myceliophthora thermophila xylanase through signal peptide engineering, resulting in enhanced enzyme activity. Further analysis showed that Mtxyn11C was more effective than commercial xylanase in degrading xylan, and when combined with other xylanolytic enzymes, it improved the hydrolysis efficiency of xylan.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

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Article Microbiology

Insights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis

Andrei Stecca Steindorff et al.

Summary: This study provides insights into the genetic repertoire of thermophilic fungus Humicola grisea and its potential as an enzyme producer for lignocellulose biomass deconstruction. It demonstrates the importance of carbon source and pH in regulating gene expression in this organism. The research paves the way for genetic manipulation and robust biotechnological applications of this fungus.

MICROBIOLOGY SPECTRUM (2021)

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Review Biotechnology & Applied Microbiology

What makes Komagataella phaffii non-conventional?

Oezge Ata et al.

Summary: K. phaffii, classified as a non-conventional yeast, stands out from both conventional yeast like Saccharomyces cerevisiae and other non-conventional yeasts in terms of regulatory features, transcriptional networks, and Golgi apparatus structure. Its distinct characteristics make it a promising subject for both fundamental research and industrial applications.

FEMS YEAST RESEARCH (2021)

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Review Engineering, Multidisciplinary

Cellulases: From Bioactivity to a Variety of Industrial Applications

Uroosa Ejaz et al.

Summary: Cellulases of microbial origin have diverse applications in various commercial sectors, including textile, pulp and paper, food service, brewing, and more. Technological advancements will further enhance their potential usage in the food industry.

BIOMIMETICS (2021)

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Article Biotechnology & Applied Microbiology

Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine

Liangtian Miao et al.

Summary: The study demonstrated the feasibility of producing beta-alanine from methanol using Pichia pastoris, achieving a high yield of 5.6g/L through optimizing enzyme activity and carbon source supply. This is the highest metabolite production titer reached in P. pastoris using methanol as the substrate.

BIORESOURCES AND BIOPROCESSING (2021)

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Review Food Science & Technology

Principles, drivers and opportunities of a circular bioeconomy

Abigail Muscat et al.

Summary: This study proposes five ecological principles for guiding biomass use towards a circular bioeconomy. Implementing these principles calls for fundamental changes to policies, technologies, organizations, social behavior, and markets.

NATURE FOOD (2021)

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Article Chemistry, Multidisciplinary

Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris

Xuerong Jin et al.

Summary: The study successfully developed a new method for the de novo biosynthesis of non-animal CS using engineered Pichia pastoris cells, producing 182.0 mg L-1 CSA with a sulfation degree of 4.0%. The results suggest that this approach could be used as a green route to produce other sulfated glycosaminoglycans.

GREEN CHEMISTRY (2021)

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Article Biotechnology & Applied Microbiology

Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification

Selfela Restu Adina et al.

Summary: This study reported the overexpression of a novel acidic lipase from Micrococcus in yeast for the first time. The extracellular expression of LipEMP48-D could be a potential biocatalyst in industrial fields, especially in organic synthesis, food industry, and biodiesel production.

JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY (2021)

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Review Biochemical Research Methods

Bioprocessing in the Digital Age: The Role of Process Models

Harini Narayanan et al.

BIOTECHNOLOGY JOURNAL (2020)

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Article Biotechnology & Applied Microbiology

Rationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris)

Miguel Angel Nieto-Taype et al.

MICROBIAL BIOTECHNOLOGY (2020)

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Review Cell Biology

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Mohsen Karbalaei et al.

JOURNAL OF CELLULAR PHYSIOLOGY (2020)

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Article Biotechnology & Applied Microbiology

The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2

Thomas Gassler et al.

NATURE BIOTECHNOLOGY (2020)

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Review Biotechnology & Applied Microbiology

The role of renewable chemicals and biofuels in building a bioeconomy

Anuj K. Chandel et al.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR (2020)

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Review Agricultural Engineering

Lignocellulosic bio-refinery approach for microbial 2,3-Butanediol production

Sulfath Hakkim Hazeena et al.

BIORESOURCE TECHNOLOGY (2020)

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Review Biochemical Research Methods

Renewable methanol and formate as microbial feedstocks

Charles A. R. Cotton et al.

CURRENT OPINION IN BIOTECHNOLOGY (2020)

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Article Microbiology

Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate

Nadielle Tamires Moreira Melo et al.

MICROORGANISMS (2020)

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Review Biotechnology & Applied Microbiology

Heterologous expression of lytic polysaccharide monooxygenases (LPMOs)

Yasser Gaber et al.

BIOTECHNOLOGY ADVANCES (2020)

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Review Biotechnology & Applied Microbiology

Engineering microbial pathways for production of bio-based chemicals from lignocellulosic sugars: current status and perspectives

Jean Marie Francois et al.

BIOTECHNOLOGY FOR BIOFUELS (2020)

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Review Biotechnology & Applied Microbiology

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development WithPichia PastorisCell Factory

Miguel Angel Nieto-Taype et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

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Article Agricultural Engineering

High-efficiency expression of a superior β-mannanase engineered by cooperative substitution method in Pichia pastoris and its application in preparation of prebiotic mannooligosaccharides

Zhemin Liu et al.

BIORESOURCE TECHNOLOGY (2020)

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Article Microbiology

Microbe Profile: Komagataella phaffii: a methanol devouring biotech yeast formerly known as Pichia pastoris

Lina Heistinger et al.

MICROBIOLOGY-SGM (2020)

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Review Energy & Fuels

Origin, Impact and Control of Lignocellulosic Inhibitors in Bioethanol Production-A Review

Nikki Sjulander et al.

ENERGIES (2020)

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Article Biotechnology & Applied Microbiology

Clean manufacturing powered by biology: how Amyris has deployed technology and aims to do it better

Paul Hill et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2020)

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Article Microbiology

Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase inPichia pastoris

Wancang Liu et al.

FRONTIERS IN MICROBIOLOGY (2020)

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Article Biotechnology & Applied Microbiology

Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins

Chung-Jen Chiang et al.

BIOTECHNOLOGY FOR BIOFUELS (2020)

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Article Biotechnology & Applied Microbiology

Mutation of cysteine residues increases heterologous expression of peach expansin in the methylotrophic yeast Pichia pastoris

Kaori Matsuyama et al.

PLANT BIOTECHNOLOGY (2020)

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Article Multidisciplinary Sciences

Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Claudia R. Arbeitman et al.

SCIENTIFIC REPORTS (2020)

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Review Multidisciplinary Sciences

Lactic acid production - producing microorganisms and substrates sources-state of art

Elahe Abedi et al.

HELIYON (2020)

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Review Biotechnology & Applied Microbiology

Cellulosic ethanol production: Progress, challenges and strategies for solutions

Chen-Guang Liu et al.

BIOTECHNOLOGY ADVANCES (2019)

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Article Biochemical Research Methods

Expression, purification and characterization of a recombinant antimicrobial peptide Hispidalin in Pichia pastoris

De-Mei Meng et al.

PROTEIN EXPRESSION AND PURIFICATION (2019)

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Review Biochemistry & Molecular Biology

Engineering Robust Production Microbes for Large-Scale Cultivation

Maren Wehrs et al.

TRENDS IN MICROBIOLOGY (2019)

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Article Biotechnology & Applied Microbiology

Toward the construction of a technology platform for chemicals production from methanol: d-lactic acid production from methanol by an engineered yeast Pichia pastoris

Ryosuke Yamada et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2019)

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Article Biotechnology & Applied Microbiology

Continuous operation, a realistic alternative to fed-batch fermentation for the production of recombinant lipase B from Candida antarctica under the constitutive promoter PGK in Pichia pastoris

Julia de Macedo Robert et al.

BIOCHEMICAL ENGINEERING JOURNAL (2019)

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Article Biotechnology & Applied Microbiology

Improving isobutanol production with the yeast Saccharomyces cerevisiae by successively blocking competing metabolic pathways as well as ethanol and glycerol formation

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BIOTECHNOLOGY FOR BIOFUELS (2019)

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Xylitol Production: Identification and Comparison of New Producing Yeasts

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Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production

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Glycerol metabolism of Pichia pastoris (Komagataella spp.) characterised by 13C-based metabolic flux analysis

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Process engineering for microbial production of 3-hydroxypropionic acid

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Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: A review

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Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

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Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

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Towards optimal substrate feeding for heterologous protein production in Pichia pastoris (Komagataella spp) fed-batch processes under PAOX1 control: a modeling aided approach

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Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol

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Optogenetic regulation of engineered cellular metabolism for microbial chemical production

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High level expression and purification of recombinant human serum albumin in Pichia pastoris

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Insight to Improve alpha-L-Arabinofuranosidase Productivity in Pichia pastoris and Its Application on Corn Stover Degradation

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Model-based tools for optimal experiments in bioprocess engineering

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A yeast for all seasons - Is Pichia pastoris a suitable chassis organism for future bioproduction?

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Scale-up of industrial microbial processes

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Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii

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Crude glycerol from biodiesel as a carbon source for production of a recombinant highly thermostable beta-mannanase by Pichia pastoris

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Development of a general defined medium for Pichia pastoris

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A comparative study of glycerol and sorbitol as co-substrates in methanol-induced cultures of Pichia pastoris: temperature effect and scale-up simulation

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Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway

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Physiological state as transferable operatingcriterion to improve recombinant protein production inPichia pastoris through oxygen limitation

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Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

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Systems biotechnology for protein production in Pichia pastoris

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Recombinant expression of Thermobifida fusca E7 LPMO in Pichia pastoris and Escherichia coli and their functional characterization

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Toward glycerol biorefinery: metabolic engineering for the production of biofuels and chemicals from glycerol

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Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

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Novel homologous lactate transporter improves l-lactic acid production from glycerol in recombinant strains of Pichia pastoris

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High-Level Secretory Expression and Purification of Recombinant Human Interleukin 1 Beta in Pichia pastoris

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Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

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Cultivation strategies to enhance productivity of Pichia pastoris: A review

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Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization

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Expression of feruloyl esterase A from Aspergillus terreus and its application in biomass degradation

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Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain III-focused high titer neutralizing antibodies

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Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris

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Optimization of a Multi-Stage, Multi-Subunit Malaria Vaccine Candidate for the Production in Pichia pastoris by the Identification and Removal of Protease Cleavage Sites

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2-Keto acids based biosynthesis pathways for renewable fuels and chemicals

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Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

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Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

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Metabolic engineering of Pichia pastoris for production of hyaluronic acid with high molecular weight

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An optogenetic gene expression system with rapid activation and deactivation kinetics

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Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol

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Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

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Yeast-expressed recombinant protein of the receptor-binding domain in SARS-CoV spike protein with deglycosylated forms as a SARS vaccine candidate

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Recent advances in biological production of 3-hydroxypropionic acid

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Recent advances in lactic acid production by microbial fermentation processes

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Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

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Heterologous expression and biochemical characterization of acetyl xylan esterase from Coprinopsis cinerea

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Xylanase and β-Xylosidase Production by Aspergillus ochraceus: New Perspectives for the Application of Wheat Straw Autohydrolysis Liquor

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Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste

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Expression of lignocellulolytic enzymes in Pichia pastoris

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Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

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Recent progress in synthetic biology for microbial production of C3-C10 alcohols

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Review Biotechnology & Applied Microbiology

Trends and challenges in the microbial production of lignocellulosic bioalcohol fuels

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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2010)

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Glycerol: A promising and abundant carbon source for industrial microbiology

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Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme

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Article Biotechnology & Applied Microbiology

Genome sequence of the recombinant protein production host Pichia pastoris

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Article Biotechnology & Applied Microbiology

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

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Use of biodiesel byproduct crude glycerol as the carbon source for fermentation processes by recombinant Pichia pastoris

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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2008)

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Review Biotechnology & Applied Microbiology

Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae

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Molecular characterization of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeast Pichia pastoris

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Description of Komagataella phaffii sp nov and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella

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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (2005)

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Review Microbiology

Heterologous protein expression in the methylotrophic yeast Pichia pastoris

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