期刊
LAB ON A CHIP
卷 22, 期 24, 页码 4882-4893出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2lc00737a
关键词
-
类别
资金
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- NIH MIRA Award [1R35GM142817]
- W.M. Keck Foundation medical research grant
- CCTSI New Methods Development pilot grant
- Colorado State University College of Veterinary Medicine and Biomedical Sciences
- CCTSI NIH-NCATS CTSA [UL1TR002535]
Caenorhabditis elegans is an important genetic model for neuroscience studies, but most studies are limited in obtaining both high-resolution microscopy imaging and behavior analysis in the same set of animals. In this paper, a novel microfluidic device using surface acoustic waves is presented to temporarily immobilize worms, allowing for non-invasive analysis of behavior and high-resolution imaging. This device enables longitudinal analysis of animal motility and subcellular morphological changes during development and aging.
Caenorhabditis elegans is an important genetic model for neuroscience studies, used for analyses of how genes control connectivity, neuronal function, and behavior. To date, however, most studies of neuronal function in C. elegans are incapable of obtaining microscopy imaging with subcellular resolution and behavior analysis in the same set of animals. This constraint stems from the immobilization requirement for high-resolution imaging that is incompatible with behavioral analysis using conventional immobilization techniques. Here, we present a novel microfluidic device that uses surface acoustic waves (SAW) as a non-contact method to temporarily immobilize worms for a short period (30 seconds). We optimize the SAW based protocol for rapid switching between free-swimming and immobilized states, facilitating non-invasive analysis of swimming behavior as well as high-resolution synaptic imaging in the same animal. We find that the coupling of heat and acoustic pressure play a key role in the immobilization process. We introduce a proof-of-concept longitudinal study, illustrating that the device enables repeated imaging of fluorescently tagged synaptic receptors in command interneurons and analysis of swimming behavior in the same animals for three days. This longitudinal approach provides the first correlative analysis of synaptic glutamatergic receptors and swimming behavior in aging animals. We anticipate that this device will enable further longitudinal analysis of animal motility and subcellular morphological changes during development and aging in C. elegans.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据