期刊
FRONTIERS IN PHYSIOLOGY
卷 7, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2016.00140
关键词
regenerative medicine; vascularization; mesenchymal stem cells; human gingival fibroblasts (hGFs); human dental pulp stem cells (hDPSCs); silk fibroin scaffolds; chorioallantoic membrane (CAM)
类别
资金
- Swiss National Foundation (SNSF) [31003A_135633]
- University of Zurich
- Matching Funds from the University of Zurich
- Swiss National Science Foundation (SNF) [31003A_135633] Funding Source: Swiss National Science Foundation (SNF)
Neovascularization is one of the most important processes during tissue repair and regeneration. Current healing approaches based on the use of biomaterials combined with stem cells in critical -size bone defects fail due to the insufficient implant vascularization and integration into the host tissues. Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from the chicken embryo chorioallantoic membrane into implanted silk fibroin scaffolds seeded with either human dental pulp stern cells or human gingival fibroblasts. Perfusion capacity was evaluated by non-invasive in vivo Magnetic Resonance Imaging while the number and density of blood vessels were measured by histomorphometry. Our results demonstrate that human dental pulp stem cells and gingival fibroblasts possess equal abilities in attracting vessels within silk fibroin scaffolds. Additionally, the prolonged in vitro pre -incubation period of these two cell populations favors the homogeneous distribution of vessels within silk fibroin scaffolds, which further improves implant survival and guarantees successful healing and regeneration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据