期刊
FOOD & FUNCTION
卷 13, 期 24, 页码 12953-12965出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2fo01866g
关键词
-
资金
- National Natural Science Foundation of China
- Natural Science Foundation of Jiangsu Province
- [81971427]
- [82101814]
- [82271744]
- [BK20221182]
This study investigates the potential therapeutic role of human milk-derived exosomes (HM-Exos) in a rat model of necrotizing enterocolitis (NEC) through comprehensive lncRNA and mRNA expression profiling. It is found that Pre-Exos exert a stronger effect on promoting the proliferation of intestinal epithelial cells in vivo compared to Term-Exos. Analysis reveals differentially expressed lncRNAs and mRNAs between Term-Exos and Pre-Exos, with the lncRNA-mRNA network of HM-Exos predicted to be involved in cellular proliferation pathways. This study sheds light on the important roles of human milk-derived lncRNAs and mRNAs in protecting against NEC and provides new insights into NEC development.
Necrotizing enterocolitis (NEC) is one of the most severe diseases commonly afflicting premature infants. Our previous studies suggests that human milk-derived exosomes (HM-Exos) have a potential therapeutic effect on NEC. In this study, we investigate the potentially therapeutic role of HM-Exos in an NEC animal model via comprehensive lncRNA and mRNA expression profiles. A rat model of NEC was induced through hypoxia, hypothermia and formula feeds. We extracted exosomes from the colostrum of healthy lactating mothers and identified their functions in an NEC animal model. Furthermore, high-throughput lncRNA and mRNA sequencings were explored to find the underlying mechanisms. Although both exosomes from term human breast milk (Term-Exos) and exosomes from preterm human breast milk (Pre-Exos) alleviated the severity of NEC, Pre-Exos seemed to better promote the proliferation of intestinal epithelial cells in vivo. We identified a total of 44 differentially expressed lncRNAs and 88 differentially expressed mRNAs between Term-Exos and Pre-Exos. Further GO and KEGG pathway analysis showed that the lncRNA-mRNA network of HM-Exos was associated with the JAK-STAT signaling pathway, bile secretion and the AMPK signaling pathway, which are predicted to be involved in the proliferation of cells. Therefore, this study reveals for the first time the important roles of human milk derived lncRNAs and mRNAs in protecting against necrotizing enterocolitis. These results provide new insight into the development of NEC.
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