期刊
CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY
卷 47, 期 3, 页码 260-266出版社
TERMEDIA PUBLISHING HOUSE LTD
DOI: 10.5114/ceji.2022.119625
关键词
children; polymorphism; TLR4; TLR2; urinary tract infections
类别
The study found that TLR2 and TLR4 gene polymorphisms are associated with children suffering from urinary tract infections (UTI), while urine neutrophil gelatinase-associated lipocalin (NGAL) concentration can reflect the degree of kidney tissue injury in children with urinary tract malformations.
Introduction: Toll-like receptors (TLRs) contribute to the innate immune system. They are an element of non-specific immunity, which enables organisms to react quickly to foreign antigens, without being previously exposed to them. TLRs are pattern recognition receptors. TLR gene polymorphisms are widely investigated in connection with various infections. The aims of the study were: to investigate the role of TLR2 and TLR4 polymorphisms in the course of urinary tract infections (UTIs); to test for differences in distribution of these polymorphisms between children with urinary tract malformations suffering from recurrent UTI (rUTI), children with malformations but without rUTI and healthy controls; to determine whether these polymorphisms predispose to rUTI; and to analyse how polymorphisms and urine neutro-phil gelatinase-associated lipocalin (NGAL) and interleukin 8 (IL-8) concentrations affect one another.Material and methods: The group consisted of 133 children (1-18 years old), 68 female and 65 male. The group was divided into 4 subgroups: A (rUTI with urinary tract malformations), B (urinary tract malformations without rUTI), C (rUTI) and D (healthy controls). Polymorphisms were analysed using PCR-RFLP. IL-8 and NGAL urine concentrations were established using immunoenzymatic methods.Results: TLR2 Arg753Gln and TLR4 Arg299Gly appeared significantly more often among children with rUTI. No correlation between urine IL-8 and urine NGAL and polymorphisms was found. Urine NGAL concentration was significantly higher among children with urinary tract malformations.Conclusions: TLR2 Arg753Gln and TLR4 Asp299Gly may predispose to rUTI. Urine NGAL concen-tration suggests the presence of kidney tissue injury, of varying degrees, among children with urinary tract malformations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据