4.3 Article

The impact of matrix age on intervertebral disc regeneration

期刊

BIOMATERIALS ADVANCES
卷 143, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.bioadv.2022.213192

关键词

IVD degeneration; regeneration; Decellularization; Ageing; Tissue engineering; 3D models

资金

  1. Fundacao para a Ciencia e a Tecnologia (FCT) [FCT-2020-PTDC/BTM-MAT/0438/2020, UIDB/04293/2020, EXPL/BTM-ORG/0880/2021, FCT/DL57/2016/CP1360/CT0005-JC, PD/BD/135544/2018-MF]
  2. QREN
  3. EUROSPINE/ON Foundation [20-165]
  4. i3S Sci-entific Platform (Advanced Light Microscopy (ALM), BioSciences Screening (BS), Bioimaging, and Histology and Electron Microscopy), member of the PPBI [PPBI-POCI-01-0145-FEDER-022122]

向作者/读者索取更多资源

This study evaluated the impact of extracellular matrix (ECM) donor age on intervertebral disc (IVD) regeneration/degeneration and found that younger ECM scaffolds have better repopulation efficiency and the ability to restore a functional IVD microenvironment.
With the lack of effective treatments for low back pain, the use of extracellular matrix (ECM)-based biomaterials have emerged with undeniable promise for IVD regeneration. Decellularized scaffolds can recreate an ideal microenvironment inducing tissue remodeling and repair. In particular, fetal tissues have a superior regenerative capacity given their ECM composition. In line with this, we unraveled age-associated alterations of the nucleus pulposus (NP) matrisome. Thus, the aim of the present work was to evaluate the impact of ECM donor age on IVD de/regeneration.Accordingly, we optimized an SDS (0.1 %, 1 h)-based decellularization protocol that preserves ECM cues in bovine NPs from different ages. After repopulation with adult NP cells, younger matrices showed the highest repopulation efficiency. Most importantly, cells seeded on younger scaffolds produced healthy ECM proteins suggesting an increased capacity to restore a functional IVD microenvironment. In vivo, only fetal matrices decreased neovessel formation, showing an anti-angiogenic potential.Our findings demonstrate that ECM donor age has a strong influence on angiogenesis and ECM de novo syn-thesis, opening new avenues for novel therapeutic strategies for the IVD. Additionally, more appropriate 3D models to study age-associated IVD pathology were unveiled.

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