Tick feeding modulates the human skin immune landscape to facilitate tick-borne pathogen transmission

During cutaneous tick attachment, the feeding cavity becomes a site of transmission for tick salivary compounds and tick-borne pathogens. However, the immunological consequences of tick feeding for human skin remain unclear. Here, we assessed human skin and blood samples upon tick bite and developed a human skin explant model mimicking Ixodes ricinus bites and tick-borne pathogen infection. Following tick attachment, we observed rapidly occurring patterns of immunomodulation, including increases in neutrophils and cutaneous B and T cells. T cells upregulated tissue residency markers, while lymphocytic cytokine production was impaired. In early stages of Borrelia burgdorferi model infections, we detected strain-specific immune responses and close spatial relationships between macrophages and spirochetes. Preincubation of spirochetes with tick salivary gland extracts hampered accumulation of immune cells and increased spirochete loads. Collectively, we showed that tick feeding exerts profound changes on the skin immune network that interfere with the primary response against tick-borne pathogens.

Automated summary

This study evaluated the immunological consequences of tick feeding on human skin and developed a human skin explant model to simulate tick bites and tick-borne pathogen infection. The results showed rapid patterns of immunomodulation upon tick attachment, as well as strain-specific immune responses and close relationships between macrophages and spirochetes. The study also found that preincubation of spirochetes with tick salivary gland extracts inhibited immune cell accumulation and increased spirochete loads.