4.6 Article

Regulating the activity of boronate moiety to construct fluorescent probes for the detection of ONOO-in vitro and in vivo

期刊

ANALYTICAL METHODS
卷 14, 期 48, 页码 5027-5033

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ay01727j

关键词

-

资金

  1. National Natural Science Foundation of China
  2. [82030107]

向作者/读者索取更多资源

In this study, a fluorescent probe based on a self-immolation mechanism was designed to detect intracellular ONOO-. It was found that p-Borate exhibited favorable selectivity and high sensitivity to ONOO- compared to other probes, and it was successfully applied to detect ONOO- in cells and inflamed mice.
Abnormal intracellular peroxynitrite (ONOO-) concentration is related to oxidative damage, which is correlated with many pathological consequences, such as local inflammation and other diseases. In this work, a series of resorufin benzyl ether-based fluorescent probes were designed using boronate as a recognizing moiety installed on a phenyl moiety for ONOO- detection via a self-immolation mechanism. The location of the boronate as well as the substitution patterns on the phenyl moiety were investigated and the responding behaviors of the designed probes to ONOO-, other reactive oxygen species, and biothiols were examined. It was found that all the immolative probes were inevitably dominated by ONOO-. Compared with other probes, p-Borate possessed favorable selectivity and high sensitivity to ONOO-. Moreover, p-Borate was successfully used to detect ONOO- in cells and inflamed mice.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据