4.6 Article

Whole-exome sequencing of selected bread wheat recombinant inbred lines as a useful resource for allele mining and bulked segregant analysis

期刊

FRONTIERS IN GENETICS
卷 13, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2022.1058471

关键词

wheat; exome capture; target-enrichment; recombinant inbred lines; bulked segregant analysis (BSA); single nucleotide polymorphisms (SNPs)

资金

  1. Ministry of Agricultural, Food and Forestry Policies (MIPAAF)
  2. European Union Next-Generation EU within the Agritech National Research Center [CN00000022]

向作者/读者索取更多资源

By combining sequence capture and target-enrichment methods with high-throughput NGS re-sequencing, we were able to scan a population of 46 randomly selected bread wheat individuals at exome-wide level and identify a large number of SNPs. This study provides an important resource for studying bread wheat genetics and improving agronomic traits through breeding programs.
Although wheat (Triticum aestivum L.) is the main staple crop in the world and a major source of carbohydrates and proteins, functional genomics and allele mining are still big challenges. Given the advances in next-generation sequencing (NGS) technologies, the identification of causal variants associated with a target phenotype has become feasible. For these reasons, here, by combining sequence capture and target-enrichment methods with high-throughput NGS re-sequencing, we were able to scan at exome-wide level 46 randomly selected bread wheat individuals from a recombinant inbred line population and to identify and classify a large number of single nucleotide polymorphisms (SNPs). For technical validation of results, eight randomly selected SNPs were converted into Kompetitive Allele-Specific PCR (KASP) markers. This resource was established as an accessible and reusable molecular toolkit for allele data mining. The dataset we are making available could be exploited for novel studies on bread wheat genetics and as a foundation for starting breeding programs aimed at improving different key agronomic traits.

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