期刊
EMERGING MICROBES & INFECTIONS
卷 5, 期 -, 页码 -出版社
TAYLOR & FRANCIS LTD
DOI: 10.1038/emi.2016.114
关键词
antibody; enzyme-linked immunosorbent assay; MERS-CoV; patient; recombinant structural antigens
资金
- Megaproject for Infectious Disease Research of China [2014ZX10004001-002]
- National Basic Research Program of China (973 Program) [2011CB504704]
- National Key Plan for Scientific Research and Development of China [2016YFD0500301]
The first imported case of Middle East respiratory syndrome (MERS) in China recently occurred, allowing for the characterization of antibody titers in a series of the patient's sera using the following methods based on recombinant viral structural antigens: inactivated MERS coronavirus (MERS-CoV) enzyme-linked immunosorbent assay (ELISA), recombinant MERS-CoV spike (S, or fragments of S) ELISA, nucleoprotein (NP) ELISA and MERS S pseudovirus particle-based neutralization test (ppNT). A longitudinal profile of the infection showed that seroconversion detected by ELISAs based on the recombinant extracellular domain, S, S1 and receptor-binding domain (RBD) antigens occurred as early as neutralizing antibodies were detected by the ppNT and earlier than antibodies were detected by the inactivated MERS-CoV and N-terminal domain (NTD) ELISAs. Antibodies detected by the NP ELISA occurred last. Strong correlations were found between the S1, RBD and NP ELISAs and the inactivated MERS-CoV ELISA. The S and RBD ELISAs were highly correlated with the commercial S1 ELISA. The S ELISA strongly correlated with the ppNT, although the MERS-CoV, S1, NTD and RBD ELISAs were also significantly correlated with the ppNT (P < 0.001).
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