期刊
KOREAN JOURNAL OF PAIN
卷 35, 期 4, 页码 423-432出版社
KOREAN PAIN SOC
DOI: 10.3344/kjp.2022.35.4.423
关键词
Amygdala; Glucocorticoids; MicroRNAs; Models; Animal; Pain Perception; Pain; Postoperative; Physiopathology; Receptors; Opioid; mu; Stress; Physiological
资金
- Postdoctoral Research Foundation of China [2018M643893]
- National Natural Science Foundationof China (NSFC) [62076253]
This study investigated the effects of perioperative stress on MOR and its mechanism, and found that miR-339-5p down-regulated MOR expression by targeting oprm1. This finding is of great significance for understanding the molecular mechanism of chronic postoperative pain.
Background: The decreased expression of mu-opioid receptors (MOR) in the amygdala may be a key molecular in chronic post-surgical pain (CPSP). It is known that miR-339-5p expression in the amygdala of a stressed rat model was increased. Analyzed by RNAhybrid, miR-339-5p could target opioid receptor mu 1 (oprm1) which codes MOR directly. So, the authors hypothesized that miR-339-5p could regulate the expression of MOR via targeting oprm1 and cause the effects to CPSP. Methods: To simulate perioperative short-term stress, a perioperative stress prolongs incision-induced pain hypersensitivity without changing basal pain perception rat model was built. A pmiR-RB-REPORT (TM) dual luciferase assay was taken to verify whether miR-339-5p could act on oprm1 as a target. The serum glucocorticoid level of rats was test. Differential expressions of MOR, GFAP, and pERK1/2 in each group of the rats' amygdala were tested, and the expressions of miR-339-5p in each group of rats' amygdalas were also measured. Results: Perioperative stress prolonged the recovery time of incision pain. The expression of MOR was down-regulated in the amygdala of rats in stress + incision (S + IN) group significantly compared with other groups (P < 0.050). miR-339-5p was up-regulated in the amygdala of rats in group S + IN significantly compared with other groups (P < 0.050). miR-339-5p acts on oprm1 3'UTR and take MOR mRNA as a target. Conclusions: Perioperative stress could increase the expression of miR-339-5p, and miR-339-5p could cause the expression of MOR to decrease via targeting oprm1. This regulatory pathway maybe an important molecular mechanism of CPSP.
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