Sequence-Based Identification of Metronidazole-Resistant Clostridioides difficile Isolates

The plasmid pCD-METRO confers metronidazole re-sistance in Clostridioides difficile. We showed high se-quence similarity among pCD-METRO plasmids from different isolates and identified pCD-METRO and as-sociated metronidazole-resistant isolates in clinical and veterinary reservoirs in the Americas. We recommend using PCR or genomic assays to detect pCD-METRO in metronidazole-resistant C. difficile.

Automated summary

The plasmid pCD-METRO is responsible for metronidazole resistance in different isolates of Clostridioides difficile. We found high sequence similarity among pCD-METRO plasmids from various sources and identified pCD-METRO and associated metronidazole-resistant isolates in clinical and veterinary reservoirs in the Americas. We suggest using PCR or genomic assays to detect pCD-METRO in metronidazole-resistant C. difficile.