In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra

The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27 degrees C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 mu mol L-1 BAP show the best combination for the in vitro propagation of D. nigra.

Automated summary

The objective of this study was to establish an efficient protocol for in vitro organogenesis of Dalbergia nigra. It was found that explants from 30-day-old cotyledonary nodes showed a higher morphogenetic potential, and the medium supplemented with 2.22 μmol/L BAP was the best combination for in vitro propagation of D. nigra.