期刊
CURRENT ISSUES IN MOLECULAR BIOLOGY
卷 44, 期 12, 页码 5986-5994出版社
MDPI
DOI: 10.3390/cimb44120407
关键词
esculetin; tert-butyl hydroperoxide; reactive oxygen species; kidney dysfunction
资金
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) - Ministry of Agriculture, Food and Rural Affairs
- [1210400202HD020]
Esculetin, derived from coumarin, has been found to have antioxidant and anti-apoptotic effects. It improves cell viability and reduces apoptosis in HEK293 cells under t-BHP-induced oxidative stress. At the molecular level, esculetin regulates the expression of pro-apoptotic and anti-apoptotic factors.
Esculetin is an antioxidant and anti-inflammatory compound derived from coumarin. Oxidative stress can cause overproduction of reactive oxygen species (ROS), which can lead to the development of chronic kidney failure. In this study, human embryonic kidney 293 (HEK293) cells were treated with tert-butyl hydroperoxide (t-BHP) to determine the antioxidant effects of esculetin. HEK293 cells were treated with t-BHP to validate changes in cell viability, ROS production, and apoptosis, and then treated with esculetin to evaluate the changes. Changes in mRNA and protein levels were analyzed using a proteome kit, PCR, and Western blotting. Esculetin improved HEK293 cell viability and reduced apoptosis caused by t-BHP-induced oxidative stress. At the mRNA and protein levels, esculetin decreased pro-apoptotic factor expression as well as increased anti-apoptotic factor expression. The antioxidant efficacy of esculetin was validated when it inhibited the apoptosis caused by t-BHP-induced oxidative stress in HEK293 cells.
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