期刊
INFECTIOUS MICROBES & DISEASES
卷 4, 期 4, 页码 175-177出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/IM9.0000000000000105
关键词
MERS-CoV; rapid antigen detection assay; dromedary camels
资金
- Health and Medical Research Fund-Commissioned Research on Control of Infectious Diseases (Phase IV) [CID-HKU6]
- Ministry of Education in Taiwan [MOE-111-S-023-A]
MERS-CoV infection in dromedaries is usually subclinical, but they serve as the main source of human infections. Rapid diagnosis of MERS-CoV in dromedaries is important for preventing transmission to humans, and a previously reported antigen detection assay showed high specificity and sensitivity.
Middle East respiratory syndrome coronavirus (MERS-CoV) infection in humans has a high mortality of >30%. Dromedaries are the reservoir of MERS-CoV and the main source of human infections. However, MERS-CoV infections in dromedaries are usually subclinical. Rapid diagnosis of MERS-CoV infection in these animals is important in preventing camel-to-human transmission of the virus. The possible cross-reactivity of a previously reported rapid nucleocapsid protein-based antigen detection assay for MERS-CoV was examined with different CoVs, including Tylonycteris bat CoV HKU4, dromedary camel CoV UAE-HKU23, human CoV-229E, human CoV-OC43, severe acute respiratory syndrome CoV-2 and rabbit CoV HKU14, where none of them showed false-positive results. The assay was further validated using quantitative real-time reverse transcription-polymerase chain reaction-confirmed MERS-CoV-positive and MERS-CoV-negative dromedary nasal samples collected in Dubai, the United Arab Emirates, which showed that the rapid antigen detection assay has a specificity of 100% and sensitivity of 91.7%.
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