Multiphoton scanning laser microscope based on a femtosecond fiber laser

We present a multiphoton scanning laser microscope based on a femtosecond frequency-doubled erbium-doped fiber laser. The laser used in the epi-illumination microscope setup generated 95 fs pulses at the wavelength of 780 nm with 44.3 mW average power at 100 MHz pulse repetition rate. The imaging process was controlled by custom software developed in the NI LabVIEW environment. Detection of two-photon fluorescence was proven by acquiring a series of images from various biological samples.

Automated summary

A multiphoton scanning laser microscope based on a femtosecond frequency-doubled erbium-doped fiber laser is presented. The microscope setup generated 95 fs pulses at 780 nm wavelength with an average power of 44.3 mW and a pulse repetition rate of 100 MHz. The imaging process was controlled by custom software developed in the NI LabVIEW environment. Two-photon fluorescence detection was proven by acquiring a series of images from various biological samples.