Engineered hydrogels for mechanobiology

Hydrogels are used to mimic cells' local environment, enabling the study of cellular responses to biochemical and mechanical cues. Here, Blache et al. discuss the challenges of creating hydrogels for mechanobiology studies and how they can be used to analyse cell behaviour in the context of mechanobiological processes and harnessed to create regenerative therapies. Cells' local mechanical environment can be as important in guiding cellular responses as many well-characterized biochemical cues. Hydrogels that mimic the native extracellular matrix can provide these mechanical cues to encapsulated cells, allowing for the study of their impact on cellular behaviours. Moreover, by harnessing cellular responses to mechanical cues, hydrogels can be used to create tissues in vitro for regenerative medicine applications and for disease modelling. This Primer outlines the importance and challenges of creating hydrogels that mimic the mechanical and biological properties of the native extracellular matrix. The design of hydrogels for mechanobiology studies is discussed, including the appropriate choice of cross-linking chemistry and strategies to tailor hydrogel mechanical cues. Techniques for characterizing hydrogels are explained, highlighting methods used to analyse cell behaviour. Example applications in regenerative medicine and for studying fundamental mechanobiological processes are provided, along with a discussion of the limitations of hydrogels as mimetics of the native extracellular matrix. The Primer ends with an outlook for the field, focusing on emerging technologies that will enable new insights into mechanobiology and its role in tissue homeostasis and disease.

Automated summary

This article discusses the challenges of creating hydrogels for mechanobiology studies and how they can be used to analyze cell behavior and create regenerative therapies. Hydrogels that mimic the mechanical properties of the extracellular matrix can provide mechanical cues to cells, allowing for the study of their impact on cellular behaviors. In addition, these hydrogels can be used to create tissues in vitro for regenerative medicine applications. The article also highlights the importance of choosing appropriate cross-linking chemistry and strategies to tailor hydrogel mechanical cues, as well as techniques for characterizing hydrogels and analyzing cell behavior.