期刊
JOURNAL OF CANCER RESEARCH AND THERAPEUTICS
卷 18, 期 6, 页码 1658-1665出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/jcrt.JCRT_1062_20
关键词
Antioxidant activity; ionizing radiation; lung toxicity; protective effect; pycnogenol
类别
资金
- Erciyes University-The Scientific Research Projects of Turkey (ERUBAP) [TSD-12-3936]
This study aims to evaluate the effects of whole-body gamma irradiation on rat lungs and the protective effect of pycnogenol. Results demonstrate that pycnogenol significantly reduces radiation-induced lung injury and decreases DNA damage and oxidative stress levels.
Background and Objective: The study aims to evaluate the histopathological changes, enzymatic alterations, and DNA damage in rat lungs induced by whole-body gamma irradiation as well as evaluation of the protective effect of pycnogenol. Materials and Methods: A hundred adult male rats were equally divided into ten groups including control, four antioxidants,gamma-irradiation, four antioxidant +gamma-irradiations. This study began the day before radiation treatment and continued for 3 days. The pycnogenol was dissolved 5% dimethyl sulfoxide and then administered orally through a gastric tube at a dose of 37.5 mg/kg, 75 mg/kg, 150 mg/kg, and 300 mg/kg in 24, 48, and 72 h before irradiation. Irradiation was applied with a whole-body irradiation dose of 900 cGy in one fraction. DNA damage, histopathological changes, catalase (CAT), and superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in lung tissue of rats were evaluated 3 days after irradiation. Results: CAT and SOD activities were found to be significantly lower in the irradiation group than control (P < 0.001). CAT and SOD activities were higher in the antioxidant + gamma-irradiation group than both irradiation and control groups. MDA levels were significantly higher in the irradiation group compared to control (P < 0.001), whereas MDA levels decreased in the antioxidant + gamma-irradiation group compared to the irradiation group. The antioxidant groups were significantly increased comet parameters depend on pycnogenol doses compared to control. The antioxidant + gamma-irradiation was decreased comet parameter compared to gamma-irradiation. As a result of the histopathologically, the antioxidant groups were different than the control group that in the areas of alveolar sacs and connective tissue areas were seen hemorrhage areas similar to the irradiation group. Conclusion: We demonstrate that 300 mg/kg of pycnogenol might provide significant protection against deleterious effects from whole-body ionizing radiation on the lung tissue. P-300+gamma-ray group was significantly reduced radiation-induced lung injury and was possible to observe significantly preservation.
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