期刊
SCIENCE TRANSLATIONAL MEDICINE
卷 14, 期 670, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
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资金
- Parker Institute for Cancer Immunotherapy
- NIH-NCI Cancer Moonshot Immuno-Oncology Translational Network Center Grant [U54 CA244438-01]
- UCSF Helen Diller Family Comprehensive Cancer Center
- NIH Director's New Innovator Award [DP2 CA239143]
- Cancer Research UK
- Kleberg Foundation
- Burroughs Welcome Fund, Career Award for Medical Scientists
- Cancer Research Institute Lloyd J. Old STAR Grant
- Jane Coffin Childs Memorial Fund Fellowship
- Diabetes, Endocrinology & Metabolism Training Grant
- NIH NIAID Training Grant
- Graduate Women In Science (GWIS) National Fellowship
- Sean Parker Autoimmunity Research Lab
- NIH [R01AR071522, R01AI136972, R01HG011239]
- DRC Center Grant [NIH P30 DK063720]
CAR Pooling is a multiplexed approach to rapidly identify CAR designs with clinical potential by evaluating the functionality of CARs with different signaling domains under prolonged antigen stimulation, aiming to improve the efficacy of CAR T cell therapies.
Chimeric antigen receptors (CARs) repurpose natural signaling components to retarget T cells to refractory cancers but have shown limited efficacy in persistent, recurrent malignancies. Here, we introduce CAR Pooling, a multiplexed approach to rapidly identify CAR designs with clinical potential. Forty CARs with signaling domains derived from a range of immune cell lineages were evaluated in pooled assays for their ability to stimulate critical T cell effector functions during repetitive stimulation that mimics long-term tumor antigen exposure. Several domains were identified from the tumor necrosis factor (TNF) receptor family that have been primarily associated with B cells. CD40 enhanced proliferation, whereas B cell-activating factor receptor (BAFF-R) and transmembrane activator and CAML interactor (TACI) promoted cytotoxicity. These functions were enhanced relative to clinical benchmarks after prolonged antigen stimulation, and CAR T cell signaling through these domains fell into distinct states of memory, cytotoxicity, and metabolism. BAFF-R CAR T cells were enriched for a highly cytotoxic transcriptional signature previously associated with positive clinical outcomes. We also observed that replacing the 4-1BB intracellular signaling domain with the BAFF-R signaling domain in a clinically validated B cell maturation antigen (BCMA)-specific CAR resulted in enhanced activity in a xeno-transplant model of multiple myeloma. Together, these results show that CAR Pooling is a general approach for rapid exploration of CAR architecture and activity to improve the efficacy of CAR T cell therapies.
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