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Article
Multidisciplinary Sciences
Kayeong Lim et al.
Summary: The researchers propose a novel base editing platform that enables targeted C-to-T base conversions in human cells without inducing insertions and deletions. The platform, called zinc finger deaminases (ZFDs), consists of custom-designed zinc-finger DNA-binding proteins, the split interbacterial toxin deaminase DddA(tox), and a uracil glycosylase inhibitor (UGI). By assembling plasmids encoding ZFDs using publicly available zinc finger resources, base editing can be achieved at high frequencies in both nuclear DNA and mtDNA. Unlike CRISPR-derived base editors, ZFDs do not cleave DNA and therefore do not produce unwanted indels at target sites. Additionally, recombinant ZFD proteins expressed in and purified from E. coli can penetrate cultured human cells spontaneously, demonstrating the proof-of-principle of gene-free gene therapy.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Pedro Silva-Pinheiro et al.
Summary: Mutations in mitochondrial DNA can lead to clinically heterogeneous diseases. Here the authors demonstrate in vivo base editing of mouse mitochondrial DNA in a post-mitotic tissue by AAV delivery of DddA-derived cytosine base editor (DdCBE).
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Sung-Ik Cho et al.
Summary: Mitochondrial DNA editing is crucial for modeling mitochondrial genetic disorders and potential future treatments. This study presents a new editing enzyme called TALEDs, which efficiently induce A-to-G editing in human mitochondria.
Article
Biotechnology & Applied Microbiology
Beverly Y. Mok et al.
Summary: Evolved DddA variants enhance the editing efficiency and expand the target compatibility of the all-protein base editor DdCBE, allowing efficient editing at non-T(C) target sites and broadening the sequence compatibility for mitochondrial and nuclear base editing.
NATURE BIOTECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Jiayin Guo et al.
Summary: The discovery of DddA-derived cytosine base editor (DdCBE) enables precise manipulation of mtDNA, holding potential for the treatment of mitochondrial disorders. In vivo testing using mouse cells and embryos demonstrated the effectiveness and accuracy of DdCBE in editing mtDNA.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Multidisciplinary Sciences
Young Geun Mok et al.
Summary: This study presents non-toxic, full-length variants of DddAtox, enabling the use of monomeric DdCBEs for efficient editing of mitochondrial DNA. The monomeric DdCBEs showed different mutation patterns compared to conventional dimeric DdCBEs and allowed base editing at sites with only one TALE protein. Additionally, transfecting mRNA encoding mDdCBEs reduced off-target editing in human mitochondrial DNA.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Hyunji Lee et al.
Summary: This study demonstrates highly efficient mitochondrial DNA editing using DdCBEs in mouse embryos, creating mitochondrial disease models and showing the potential for treating mitochondrial disorders. The authors show the use of split DddA-derived base editors fused to TALEs in mouse embryos with germline transmission.
NATURE COMMUNICATIONS
(2021)
Letter
Biochemistry & Molecular Biology
Riqing Li et al.
Article
Plant Sciences
Issei Nakazato et al.
Summary: By fusing bacterial cytidine deaminase with the DNA binding domains of transcription activator-like effector nucleases, targeted base editing in the plastid genome of Arabidopsis thaliana was achieved, with mutations inherited by offspring independently of their nuclear-introduced vectors.
Article
Plant Sciences
Beum-Chang Kang et al.
Summary: A new Golden Gate cloning system was developed for gene editing in plant organelles, enabling efficient point mutation editing in mitochondria and chloroplasts. This system achieved high frequencies of base editing in plant calli and plantlets, providing a promising approach for genetic manipulation in plants.
Review
Plant Sciences
Jedrzej Dobrogojski et al.
ACTA PHYSIOLOGIAE PLANTARUM
(2020)
Article
Multidisciplinary Sciences
Beverly Y. Mok et al.
Review
Genetics & Heredity
Payam A. Gammage et al.
TRENDS IN GENETICS
(2018)
Article
Multidisciplinary Sciences
Kentaro Miyazaki et al.
SCIENTIFIC REPORTS
(2018)
Editorial Material
Plant Sciences
Tetsuya Ishii
TRENDS IN PLANT SCIENCE
(2018)
Letter
Biotechnology & Applied Microbiology
Jungeun Kim et al.
NATURE BIOTECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Yongsub Kim et al.
NATURE BIOTECHNOLOGY
(2013)
Article
Biochemical Research Methods
Heng Li et al.
Article
Biochemical Research Methods
Xiuren Zhang et al.
