期刊
NANO RESEARCH
卷 -, 期 -, 页码 -出版社
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-022-5328-9
关键词
nanozymes; peroxidase mimics; Au@MoSe2 nanostructures; total antioxidant capacity assay
类别
资金
- National Natural Science Foundation of China
- Hunan Provincial Natural Science Foundation of China
- [21804050]
- [22004032]
- [2021JJ20020]
A visual colorimetric detection strategy based on gold nanoparticle-decorated MoSe2 nanosheets was reported for total antioxidant capacity (TAC) assay. This method showed high sensitivity for ascorbic acid (AA) detection and was successfully applied in commercial beverages and vitamin C tablets.
A visual colorimetric detection strategy is reported for total antioxidant capacity (TAC) assay by using 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) oxidation as chromogenic substrate based on gold nanoparticle-decorated MoSe2 nanosheets (Au@MoSe2). Au@MoSe2 nanostructures exhibit high peroxidase-like activity and can catalyze H2O2 to oxidize TMB. Based on inhibition effect of ascorbic acid (AA) on TMB oxidation, a facile and sensitive colorimetric method was developed for AA detection. Under optimal conditions, the proposed method showed a sensitivity for AA in a concentration range from 2 to 120 mu M and limit of detection was 0.41 mu M. Furthermore, the method was employed for TAC assay in actual samples, including commercial beverages and vitamin C tablets. This work represents a model in nanostructure design and will lead to further development of TAC assay in evaluation of antioxidant food quality.
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