期刊
ACS SYNTHETIC BIOLOGY
卷 11, 期 9, 页码 2909-2916出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.2c000792909
关键词
chassis; biosensor; environmental; synthetic biology; marine; soil
资金
- Moore Foundation [7524]
- Office of Naval Research (ONR) [N00014-21-1-2362]
Microbial biosensors play a crucial role in sensing and reporting exposures to stimuli, aiding our understanding of environmental processes. However, selecting suitable microbial hosts, or chassis, for biosensors is a challenge. This study evaluates the ecological, metabolic, and genetic constraints for chassis development and proposes a conceptual framework for selecting environmental biosensor chassis.
Microbial biosensors sense and report exposures to stimuli, thereby facilitating our understanding of environmental processes. Successful design and deployment of biosensors hinge on the persistence of the microbial host of the genetic circuit, termed the chassis. However, model chassis organisms may persist poorly in environmental conditions. In contrast, non-model organisms persist better in environmental conditions but are limited by other challenges, such as genetic intractability and part unavailability. Here we identify ecological, metabolic, and genetic constraints for chassis development and propose a conceptual framework for the systematic selection of environmental biosensor chassis. We identify key challenges with using current model chassis and delineate major points of conflict in choosing the most suitable organisms as chassis for environmental biosensing. This framework provides a way forward in the selection of biosensor chassis for environmental synthetic biology.
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