4.8 Article

Label-Free Electrochemical Immunosensor Made with Tree-like Gold Dendrites for Monitoring 25-Hydroxyvitamin D3 Metabolite

期刊

ACS APPLIED MATERIALS & INTERFACES
卷 14, 期 27, 页码 31455-31462

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c0838131455

关键词

25-hydroxyvitamin D3 monitoring; personalized nutrition; flexible sensor; tree-like gold nanostructures; electrochemical immunosensor

资金

  1. Brazilian agencies: Coordination of Superior Level Staff Improvement (CAPES) [001, 88887.334689/2019-00]
  2. Sao Paulo Research Foundation (FAPESP) [2019/13514-9, 2019/01777-5, 2018/22214-6]
  3. National Council for Scientific and Technological Development (CNPq)

向作者/读者索取更多资源

This article introduces a flexible and fully printed immunosensor with optimized molecular structures and working conditions, which achieves high sensitivity for metabolite detection. The sensor is stable, reproducible, and unresponsive to interference from other molecules. It can also be used as a wearable sensor with mechanical flexibility and customization.
Flexible, fully printed immunosensors can meet the requirements of precision nutrition, but this demands optimized molecular architectures to reach the necessary sensitivity. Herein, we report on flexible and label-free immunosensor chips made with tree-like gold dendrites (AuDdrites) electrochemically formed by selective desorption of L-cysteine (L-cys) on (111) gold planes. Electrodeposition was used because it is scalable and cost-effective for a rapid, direct growth of Au hyperbranched dendritic structures. The 25-hydroxyvitamin D3 (25(OH)D3) metabolite was detected within 15 min with a limit of detection (LOD) of 0.03 ng mL(-1). This high performance was possible due to the careful optimization of the electroactive layer and working conditions for square wave voltammetry (SWV). Electrocrystallization was manipulated by controlling the deposition potential and the molar ratio between HAuCl4 and L-cys. Metabolite detection was performed on human serum and saliva samples with adequate recovery between 97% and 100%. The immunosensors were stable and reproducible, unresponsive to interference from other molecules in human serum and saliva. They can be extended for use as wearable sensors with their mechanical flexibility and possible customization.

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