rs5745676 of HGF 3′UTR associates with hsa-miR-340-5p binding potential in breast cancer and gastric cancer in Isfahan population

Introduction: History of Family is a vital risk issue in breast cancer. Moreover, gastric cancer is classified as a multifactorial disease that is caused by various factors such as infectious, environmental, and genetic factors in individuals. Hepatocyte growth factor (HGF) is located on chromosome 7q21.11 HGF could be bound to the c-Met receptor and might be activated the tyrosine kinase axis. MicroRNAs have a length of 18-25 nucleotides. SNP is a genetic change that occurs when a nucleotide changes in the genome sequence. Materials and methods: 129 breast cancer patients and 132 healthy subjects. Besides, 136 gastric cancer patients and 50 controls were enrolled. We evaluated the diagram using MICPCR software using a high-temperature melting curve diagram and then predicted functions of rs5745676 via bioinformatics. Results: rs5745676 of HGF gene with AA, AG, and GG genotypes were observed in the study population. This study revealed the Estrogen receptor, Progesterone receptor, Human epidermal growth factor receptor 2, metastasis, and cancer grade were associated with genotypes in breast cancer. In addition, the blood group was significantly associated with genotypes in gastric cancer patients. Discussion: It was anticipated that the AG genotype and G allele could be considered a risk in breast cancer and also gastric cancer. Since this SNP has the potential to prime cancer progression signaling pathways by modifying the binding ability of miR-340-5p to HGF 3'UTR.

Automated summary

Family history is an important risk factor for breast cancer, and gastric cancer is a multifactorial disease. The rs5745676 of the HGF gene has been found to be associated with the risk of both breast and gastric cancer.