期刊
STEM CELLS INTERNATIONAL
卷 2016, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2016/7419389
关键词
-
资金
- Shanghai Jiao Tong University Fund [14X130040002, AE1700019]
- National Natural Science Foundation of China [81270432]
Alzheimer's disease, one of the neurodegenerative diseases, shows the progressive senescence of neural progenitor/stem cells. N-Stearoyl-L-tyrosine (NsTyr) showed neuroprotective effect against chronic brain ischemia in previous reports. In the present study, we find the antisenescent effects of NsTyr-2K in NSPCs induced by A beta(1-42) in vitro. Cell viability of NSPCs was evaluated by CCK8 assay; SA-beta-gal staining was used to evaluate senescence of NSPCs. CB receptors were detected by immunohistochemistry in NSPCs. AM251 or AM630 was used to offset the anti-senescence effects afforded by NsTyr-2K. The positive rate of SA-beta-gal staining was significantly increased in NSPCs after incubation with A beta(1-42) for 9 days. CB receptors were found on the surface of NSPCs. The expression level of CB1 receptors was significantly decreased in NSPCs after incubation with A beta(1-42). This phenomenon was reversed dose-dependently by NsTyr-2K. NsTyr-2K attenuated A beta(1-42) induced NSPCs senescence dose-dependently, and its antisenescence effect was completely abolished by AM630. A beta(1-42) dose-dependently increased the prosenescence molecules p16 and Rb. Their expression was inhibited by NsTyr-2K dose-dependently and blocked by AM630 in NSPCs. These results suggest that NsTyr-2K can alleviate the senescence of NSPCs induced by A beta(1-42) via CB2 receptor.
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