期刊
CELL REPORTS METHODS
卷 2, 期 11, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.crmeth.2022.100324
关键词
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资金
- National Key R&D Program of China [2021YFA1301201]
- Key Research and Development Plan of Shaanxi Province [2021ZDLSF01-10]
The gut microbiome is crucial for human health. This study introduces a novel model using bacteriophages to selectively deplete specific bacteria in mice, allowing for a more in-depth understanding of their functions.
The gut microbiome is essential for human health. Mouse microbiota models, including gnotobiotic mice, are the most prominent tools to elucidate the functions of gut bacteria. Here, we propose a targeted-bacterium-depleted (TBD) model using lytic bacteriophage to selectively deplete gut bacterium of healthy or otherwise defined mice. These phage-treated animals should have a near-complete spectrum of gut bacteria except for the depleted bacterium. To prove the concept, we employed Escherichia coli-specific phage T7 to repress E. coli in the healthy mice. Our results showed that the E. coli-depleted mice exhibited bravery-like behaviors, correlated to the presence of E. coli rather than the equilibrium among gut bacteria. Thus, we demonstrate that the TBD model is a powerful tool to elucidate the function of a specific bacterial species within a near-intact gut microbiota environment and complements gnotobiotic mice models.
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