期刊
SCIENCE SIGNALING
卷 9, 期 453, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scisignal.aag1625
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资金
- NIH [R37 GM048071, RO1 GM065830, F31 GM119330-01]
- UBA
- FONCyT (Argentina) [UBACyT 20020130100480BA, PICT 2013-1301]
The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca2+-liberating second messenger inositol trisphosphate (IP3) diffuses with a coefficient(similar to 280 mu m(2) s(-1)) similar to that in water, corresponding to a range of action of similar to 25 mm. Consequently, IP3 is generally considered a global cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca2+ puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (<= 10 mu m(2) s-1) about 30-fold slower than that previously reported. We propose that diffusion of IP3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP3 (< 5 mu m) is thus smaller than the size of typical mammalian cells, indicating that IP3 should better be considered as a local rather than a global cellular messenger.
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