Transcriptomic Comparison Analysis between Ameloblastoma and AM-1 Cell Line

Cancer initiation and progression are profoundly along with the crosstalk between cancer cells and the surrounding stroma. Accumulating evidence has shown that the therapy targeting the extracellular matrix (ECM) would regress tumor growth and invasion in the most common carcinomas. However, it remains largely unexplored in several rare tumors like odontogenic tumors. Ameloblastoma (AM) is the representative odontogenic epithelial tumor in the jaw-bone, and it usually infiltrates into adjacent bone marrow and has unlimited growth capacity and a high potential for recurrence. This study aims to investigate the role of collagen-rich ECM during the invasion of AM. Transcriptomic analysis revealed that ECM-and epithelial-to-mesenchymal transition (EMT)-related genes were up-regulated in AM compared to ameloblastoma cell line, AM-1. Tumoroid forming analysis showed that Collagen-rich ECM is indis-pensable for AM progression, especially for aggressive growth patterns and collective invasion.

Automated summary

Cancer initiation and progression are closely related to the interaction between cancer cells and the surrounding stroma. This study focuses on the role of collagen-rich extracellular matrix (ECM) in the invasion of ameloblastoma (AM), a representative odontogenic epithelial tumor. Transcriptomic analysis showed up-regulation of ECM- and epithelial-to-mesenchymal transition (EMT)-related genes in AM compared to the ameloblastoma cell line. Tumoroid forming analysis demonstrated the importance of collagen-rich ECM in AM progression, particularly in aggressive growth patterns and collective invasion.