期刊
JOURNAL OF TISSUE ENGINEERING
卷 13, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/20417314221143484
关键词
Elastic cartilage; rotating culture; microtia; auricular perichondrial chondroprogenitor cells; micro three-dimensional culture
资金
- Japan Agency for Medical Research and Development (AMED) [JP21lm0203122h0002]
- Terumo Life Science Foundation [19-T001]
In this study, a novel in vitro scaffold-free method for elastic cartilage reconstruction using human auricular perichondrial chondroprogenitor cells was developed. Inducing chondrogenesis with microscopic spheroids increased the chondrogenic potential. Craniofacial transplantation in immunodeficient mice showed that the reconstructed tissue was morphologically stable.
Morphologically stable scaffold-free elastic cartilage tissue is crucial for treating external ear abnormalities. However, establishing adequate mechanical strength is challenging, owing to the difficulty of achieving chondrogenic differentiation in vitro; thus, cartilage reconstruction is a complex task. Auricular perichondrial chondroprogenitor cells exhibit high proliferation potential and can be obtained with minimal invasion. Therefore, these cells are an ideal resource for elastic cartilage reconstruction. In this study, we aimed to develop a novel in vitro scaffold-free method for elastic cartilage reconstruction, using human auricular perichondrial chondroprogenitor cells. Inducing chondrogenesis by using microscopic spheroids similar to auricular hillocks significantly increased the chondrogenic potential. The size and elasticity of the tissue were maintained after craniofacial transplantation in immunodeficient mice, suggesting that the reconstructed tissue was morphologically stable. Our novel tissue reconstruction method may facilitate the development of future treatments for external ear abnormalities.
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