期刊
PATHOGENS AND DISEASE
卷 74, 期 4, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femspd/ftw023
关键词
Leishmania (V.) braziliensis; amastigote; nitric oxide; mucosal leishmaniasis; Interferon-gamma
资金
- CNPq
- CAPES
- FAPEG
Mucosal leishmaniasis (ML) caused by Leishmania (Vianna) braziliensis usually appears after the healing of the primary lesion when amastigotes disseminate from the infection site to the mucosal area. Here, we investigated murine infection with amastigotes obtained from patients with ML or localized cutaneous leishmaniasis (LCL). Amastigotes were used to infect wild type, IFN-gamma KO and inducible nitric oxide synthase (iNOS) KO mice. Amastigotes from patients with LCL induced lesions that appeared earlier in IFN-gamma KO than parasites from ML. The lesion after infection with ML appeared early in iNOS KO than in IFN-gamma KO mice and in iNOS KO mice parasites from ML and LCL cause similar lesions at the initial phase of infection, while parasites from ML induced greater lesions than the ones from LCL at the late phase. A greater number of parasites were observed in spleen of IFN-gamma KO and iNOS KO mice infected with amastigotes from patients with ML than those with LCL. Parasites from ML infect a lower percentage of macrophages and are killed independent on IFN-gamma and dependent on NO. The data suggest that amastigotes responsible for mucosal lesion in humans develop slowly on the initial phase of infection due to high susceptibility to NO and they have an increased ability to disseminate.
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