期刊
THERANOSTICS
卷 12, 期 16, 页码 6883-6897出版社
IVYSPRING INT PUBL
DOI: 10.7150/thno.77457
关键词
Molecular engineering; Semiconducting polymer nanoparticle; Afterglow imaging; Immunogenic cell death; Therapy monitoring
资金
- National Natural Science Foundation of China [U21A20287, 21977027, 21974039, 51872088, 21890744]
- Science and Technology Project of Hunan Province [2020RC3022, 2020SK2014, 2020RC3066, 2021JJ20003]
A novel near-infrared organic afterglow nanoparticle was developed to visualize reactive oxygen species (ROS) during immunogenic cell death, with high signal to noise ratio. The nanoparticle induced immunogenic cell death of cancer cells and caused immune responses, providing a non-invasive tool for predicting the degree of immunogenic cell death during ROS-mediated cancer therapy.
Rationale: Immunogenic cell death (ICD)-associated immunogenicity evoked through reactive oxygen species (ROS) is an efficient way to fight against the immune-dysfunctional microenvironment, so as to provoke potent anti-tumor immunity. However, the unknown ROS dose during cancer therapies may induce adverse immune responses (e.g., insufficient ICD, toxicity toward normal tissues or immune system). Methods: Herein, we developed a pyrido pyrazine - thiophene based semiconducting polymer as novel near-infrared ( NIR) organic afterglow nanoparticles for the real-time visualization of self-generated ROS, during photodynamic-mediated immunogenic cell death. Specifically, we introduced the strong acceptor (pyrido pyrazine) into thiophene based semiconducting polymer to redshift emission wavelength, and further modulate the donor to afford more afterglow reaction sites and reducing.Est, so as to enhance luminescence intensity. Results: The semiconducting polymer-based afterglow nanoparticles exhibit strong afterglow emission with longer-wavelength emission (> 800 nm), compared with the reported organic afterglow nanoparticles (e.g., MEHPPV, PFODBT or Chlorin, < 690 nm), which endows this afterglow nanoparticles with a greatly improvement of signal to noise ratio. Moreover, the photodynamic effect of this afterglow nanoparticles can induce immunogenic cell death of cancer cells and further cause immune responses in mice. Conclusions: The NIR afterglow signal presents a good relationship with ROS generation, immunogenic cell death and outcome of treatment. Therefore, it was able to provide a non-invasive tool for predicting the degree of ICD that occurs during ROS-mediated cancer therapy and may contribute to precise immunotherapy.
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