Measurement of Fe(II) and Fe(III) in Groundnut by In-column and Post-column Reactions in Ion Chromatography

The present communication deals with the development of a new specific, sensitive, rapid, and economical procedure for the determination of iron ions [Fe(III) and Fe(II)] from groundnut. Detection and measurement of both ions were performed in 25 mu L of hot water leaf extract of groundnut (cv. GG 7). Both ions were detected and measured via in-column and post-column reactions with salicylic acid (SA) and 1,10-phenanthroline by ion chromatography with ultraviolet-visible detection. The Fe(III) ion was detected as the complex with salicylic acid, whereas Fe(II) ion was detected as the complex with 1,10-phenanthroline at 522nm with different retention times. Results of the analysis were validated statistically and by recovery studies.